Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/91126
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Isolation, via 454 sequencing, and characterisation of microsatellites for Phalacrocorax fuscescens, the black-faced cormorant (Aves:Phalacrocoracidae)
Author: Riordan, J.
Gardner, M.
Fitch, A.
Johnston, G.
Citation: Australian Journal of Zoology, 2012; 60(5):340-342
Publisher: CSIRO Publishing
Issue Date: 2012
ISSN: 0004-959X
1446-5698
Statement of
Responsibility: 
Julie Riordan, Michael G. Gardner, Alison J. Fitch and Gregory R. Johnston
Abstract: The black-faced cormorant, Phalacrocorax fuscescens, is a brood-reducing seabird endemic to the southern waters of Australia. Microsatellite loci were isolated from genomic DNA using 454 shotgun sequencing. Thirty-one loci were tested and, of these, 16 were found to be polymorphic. Further characterisation was conducted on seven loci that were genotyped in 42 adult individuals from a single breeding colony in South Australia. The number of alleles per locus ranged from three to eight (s.d. ± 2.16), and the mean observed and expected heterozygosity was 0.66 (s.d. ± 0.249) and 0.62 (s.d. ± 0.178) respectively. We confirm that four loci conformed to Hardy–Weinberg expectations. Four other Phalacrocorax species were trialled for amplification of these four polymorphic loci. Amplification success varied between loci and species. These loci will be useful in determining genetic family structure and exploring nestling relatedness to further understand how relatedness influences competitive behaviours in brood-reducing species.
Keywords: 454 GS-FLX; shotgun sequencing
Rights: © The Authors
RMID: 0030009075
DOI: 10.1071/ZO12084
Appears in Collections:Earth and Environmental Sciences publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.