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Type: Journal article
Title: Isolation and characterisation of eighteen microsatellite markers from the sea cucumber Holothuria scabra (Echinodermata : Holothuriidae)
Author: Fitch, A.
Leeworthy, G.
Li, X.
Bowman, W.
Turner, L.
Gardner, M.
Citation: Australian Journal of Zoology, 2012; 60(6):368-371
Publisher: CSIRO Publishing
Issue Date: 2012
ISSN: 0004-959X
Statement of
Alison J. Fitch, Grant Leeworthy, Xiaoxu Li, Will Bowman, Luke Turner and Michael G. Gardner
Abstract: We isolated 18 new, polymorphic, microsatellite markers from Holothuria scabra, a commercially important species of sea cucumber found throughout the Asia-Pacific region. Next-generation sequencing was used to identify 206 unique loci for which primers were designed. Of these unique loci, we trialled 65 primer pairs in the target species, and 48 (74%) amplified a product of the expected size. Eighteen loci were found to be polymorphic and reliable, and were screened for variation in 50 individuals, from a single population from Croker Island, Northern Territory, Australia. Observed heterozygosity ranged from 0.00 to 0.96 (mean = 0.46) and the number of alleles per locus from 2 to 28 (mean = 9.61). These loci will be useful for the investigation of population structure and mating systems in H. scabra and may also be of use in other holothurian species.
Keywords: GS-FLX; next-generation sequencing
Rights: Copyright the authors
DOI: 10.1071/ZO12114
Appears in Collections:Aurora harvest 7
Earth and Environmental Sciences publications

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