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Type: Journal article
Title: Microbial cell lysis and nucleic acid extraction via nanofluidic channel
Author: Kant, K.
Yoo, J.
Amos, S.
Erkelens, M.
Priest, C.
Shapter, J.
Losic, D.
Citation: RSC Advances: an international journal to further the chemical sciences, 2015; 5(30):23886-23891
Publisher: Royal Society of Chemistry
Issue Date: 2015
ISSN: 2046-2069
Statement of
Krishna Kant, Jeongha Yoo, Steven Amos, Mason Erkelens, Craig Priest, Joe G. Shapter and Dusan Losic
Abstract: This paper presents a microfluidic device with a nano-channel prepared by focused ion beam (FIB) milling for microbial cell lysis and nucleic acid extraction. The device prepared in a quartz slide combines two microchannels connected with one nanochannel in the middle with dimensions of 100 nm (width) and 250 nm (depth) and 1 μm (length). The capturing of a single or a few cells and their lysis for extraction of DNA in nanochannel were demonstrated. The purity and quantity of the extracted genetic material was tested using a UV spectrometer. The results revealed that the extraction methodology was successful and may be well-suited to integration in analytical lab-on-a-chip devices.
DOI: 10.1039/c5ra01336d
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Chemical Engineering publications

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