Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/91370
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Type: Journal article
Title: Validation of an optimized method for the determination of iodine in human breast milk by inductively coupled plasma mass spectrometry (ICPMS) after tetramethylammonium hydroxide extraction
Author: Huynh, D.
Zhou, S.
Gibson, R.
Palmer, L.
Muhlhausler, B.
Citation: Journal of Trace Elements in Medicine and Biology, 2015; 29:75-82
Publisher: Elsevier
Issue Date: 2015
ISSN: 0946-672X
1878-3252
Statement of
Responsibility: 
Dao Huynh, Shao Jia Zhou, Robert Gibson, Lyndon Palmer, Beverly Muhlhausler
Abstract: In this study a novel method to determine iodine concentrations in human breast milk was developed and validated. The iodine was analyzed by inductively coupled plasma mass spectrometry (ICPMS) following tetramethylammonium hydroxide (TMAH) extraction at 90°C in disposable polypropylene tubes. While similar approaches have been used previously, this method adopted a shorter extraction time (1h vs. 3h) and used antimony (Sb) as the internal standard, which exhibited greater stability in breast milk and milk powder matrices compared to tellurium (Te). Method validation included: defining iodine linearity up to 200μgL(-1); confirming recovery of iodine from NIST 1549 milk powder. A recovery of 94-98% was also achieved for the NIST 1549 milk powder and human breast milk samples spiked with sodium iodide and thyroxine (T4) solutions. The method quantitation limit (MQL) for human breast milk was 1.6μgL(-1). The intra-assay and inter-assay coefficient of variation for the breast milk samples and NIST powder were <1% and <3.5%, respectively. NIST 1549 milk powder, human breast milk samples and calibration standards spiked with the internal standard were all stable for at least 2.5 months after extraction. The results of the validation process confirmed that this newly developed method provides greater accuracy and precision in the assessment of iodine concentrations in human breast milk than previous methods and therefore offers a more reliable approach for assessing iodine concentrations in human breast milk.
Keywords: Human breast milk; ICPMS; Iodine
Rights: © 2014 Elsevier GmbH. All rights reserved.
RMID: 0030010154
DOI: 10.1016/j.jtemb.2014.07.005
Grant ID: http://purl.org/au-research/grants/nhmrc/626800
Appears in Collections:Agriculture, Food and Wine publications

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