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Type: Journal article
Title: DRONC, an ecdysone-inducible Drosophila caspase
Author: Dorstyn, L.
Colussi, P.
Quinn, L.
Richardson, H.
Kumar, S.
Citation: Proceedings of the National Academy of Sciences of USA, 1999; 96(36251):4307-4312
Issue Date: 1999
ISSN: 0027-8424
Statement of
Loretta Dorstyn, Paul A. Colussi, Leonie M. Quinn, Helena Richardson and Sharad Kumar
Abstract: Caspases play an essential role in the execution of programmed cell death in metazoans. Although 14 caspases are known in mammals, only a few have been described in other organisms. Here we describe the identification and characterization of a Drosophila caspase, DRONC, that contains an amino terminal caspase recruitment domain. Ectopic expression of DRONC in cultured cells resulted in apoptosis, which was inhibited by the caspase inhibitors p35 and MIHA. DRONC exhibited a substrate specificity similar to mammalian caspase-2. DRONC is ubiquitously expressed in Drosophila embryos during early stages of development. In late third instar larvae, dronc mRNA is dramatically up-regulated in salivary glands and midgut before histolysis of these tissues. Exposure of salivary glands and midgut isolated from second instar larvae to ecdysone resulted in a massive increase in dronc mRNA levels. These results suggest that DRONC is an effector of steroid-mediated apoptosis during insect metamorphosis.
Keywords: 3T3 Cells
Embryo, Nonmammalian
Drosophila Proteins
Recombinant Proteins
Sequence Alignment
Transcription, Genetic
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
Enzyme Induction
Amino Acid Sequence
Sequence Homology, Amino Acid
Substrate Specificity
Expressed Sequence Tags
Molecular Sequence Data
Caspase 2
Description: Copyright © 1999, The National Academy of Sciences
DOI: 10.1073/pnas.96.8.4307
Published version:
Appears in Collections:Aurora harvest
Medicine publications

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