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Type: Journal article
Title: Expression of inducible heat shock proteins Hsp27 and Hsp70 in the visual pathway of rats subjected to various models of retinal ganglion cell injury
Author: Chidlow, G.
Wood, J.
Casson, R.
Citation: PLoS One, 2014; 9(12):e114838-1-e114838-26
Publisher: Public Library of Science
Issue Date: 2014
ISSN: 1932-6203
Statement of
Glyn Chidlow, John P. M. Wood, Robert J. Casson
Abstract: Inducible heat shock proteins (Hsps) are upregulated in the central nervous system in response to a wide variety of injuries. Surprisingly, however, no coherent picture has emerged regarding the magnitude, duration and cellular distribution of inducible Hsps in the visual system following injury to retinal ganglion cells (RGCs). The current study sought, therefore, to achieve the following two objectives. The first aim of this study was to systematically characterise the patterns of Hsp27 and -70 expression in the retina and optic nerve in four discrete models of retinal ganglion cell (RGC) degeneration: axonal injury (ON crush), somato-dendritic injury (NMDA-induced excitotoxicity), chronic hypoperfusion (bilateral occlusion of the carotid arteris) and experimental glaucoma. The second aim was to document Hsp27 and -70 expression in the optic tract, the subcortical retinorecipient areas of the brain, and the visual cortex during Wallerian degeneration of RGC axons. Hsp27 was robustly upregulated in the retina in each injury paradigm, with the chronic models, 2VO and experimental glaucoma, displaying a more persistent Hsp27 transcriptional response than the acute models. Hsp27 expression was always associated with astrocytes and with a subset of RGCs in each of the models excluding NMDA. Hsp27 was present within astrocytes of the optic nerve/optic tract in control rats. During Wallerian degeneration, Hsp27 was upregulated in the optic nerve/optic tract and expressed de novo by astrocytes in the lateral geniculate nucleus and the stratum opticum of the superior colliculus. Conversely, the results of our study indicate Hsp70 was minimally induced in any of the models of injury, either in the retina, or in the optic nerve/optic tract, or in the subcortical, retinorecipient areas of the brain. The findings of the present study augment our understanding of the involvement of Hsp27 and Hsp70 in the response of the visual system to RGC degeneration.
Keywords: Visual Cortex
Visual Pathways
Retinal Ganglion Cells
Optic Nerve
Rats, Sprague-Dawley
Infarction, Middle Cerebral Artery
Retinal Degeneration
Disease Models, Animal
Neurofilament Proteins
RNA, Messenger
Fluorescent Antibody Technique
Time Factors
HSP70 Heat-Shock Proteins
Superior Colliculi
HSP27 Heat-Shock Proteins
Optic Tract
Rights: © 2014 Chidlow et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: 10.1371/journal.pone.0114838
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Appears in Collections:Aurora harvest 7
Opthalmology & Visual Sciences publications

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