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Type: Journal article
Title: The Na⁺ transporter, TaHKT1;5-D, limits shoot Na⁺ accumulation in bread wheat
Other Titles: The Na(+) transporter, TaHKT1;5-D, limits shoot Na(+) accumulation in bread wheat
Author: Byrt, C.
Xu, B.
Krishnan, M.
Lightfoot, D.
Athman, A.
Jacobs, A.
Watson-Haigh, N.
Plett, D.
Munns, R.
Tester, M.
Gilliham, M.
Citation: The Plant Journal, 2014; 80(3):516-526
Publisher: Wiley
Issue Date: 2014
ISSN: 0960-7412
Statement of
Caitlin Siobhan Byrt, Bo Xu, Mahima Krishnan, Damien James Lightfoot, Asmini Athman, Andrew Keith Jacobs, Nathan S. Watson-Haigh, Darren Plett, Rana Munns, Mark Tester and Matthew Gilliham
Abstract: Bread wheat (Triticum aestivum L.) has a major salt tolerance locus, Kna1, responsible for the maintenance of a high cytosolic K(+) /Na(+) ratio in the leaves of salt stressed plants. The Kna1 locus encompasses a large DNA fragment, the distal 14% of chromosome 4DL. Limited recombination has been observed at this locus making it difficult to map genetically and identify the causal gene. Here, we decipher the function of TaHKT1;5-D, a candidate gene underlying the Kna1 locus. Transport studies using the heterologous expression systems Saccharomyces cerevisiae and Xenopus laevis oocytes indicated that TaHKT1;5-D is a Na(+) -selective transporter. Transient expression in Arabidopsis thaliana mesophyll protoplasts and in situ polymerase chain reaction indicated that TaHKT1;5-D is localised on the plasma membrane in the wheat root stele. RNA interference-induced silencing decreased the expression of TaHKT1;5-D in transgenic bread wheat lines which led to an increase in the Na(+) concentration in the leaves. This indicates that TaHKT1;5-D retrieves Na(+) from the xylem vessels in the root and has an important role in restricting the transport of Na(+) from the root to the leaves in bread wheat. Thus, TaHKT1;5-D confers the essential salinity tolerance mechanism in bread wheat associated with the Kna1 locus via shoot Na(+) exclusion and is critical in maintaining a high K(+) /Na(+) ratio in the leaves. These findings show there is potential to increase the salinity tolerance of bread wheat by manipulation of HKT1;5 genes.
Keywords: wheat; Triticum aestivum; sodium exclusion; HKT; salt tolerance; RNA interference
Rights: © 2014 The Authors The Plant Journal © 2014 John Wiley & Sons Ltd
RMID: 0030007892
DOI: 10.1111/tpj.12651
Grant ID:
Appears in Collections:Agriculture, Food and Wine publications

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