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https://hdl.handle.net/2440/91748
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dc.contributor.author | Shavrukov, Y. | - |
dc.contributor.author | Baho, M. | - |
dc.contributor.author | Lopato, S. | - |
dc.contributor.author | Langridge, P. | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Plant Biotechnology Journal, 2016; 14(1):313-322 | - |
dc.identifier.issn | 1467-7644 | - |
dc.identifier.issn | 1467-7652 | - |
dc.identifier.uri | http://hdl.handle.net/2440/91748 | - |
dc.description.abstract | Drought tolerance of the wheat cultivar Bobwhite was previously enhanced by transformation with a construct containing the wheat DREB3 gene driven by the stress-inducible maize Rab17 promoter. Progeny of a single T2 transgenic line were used as pollinators in crosses with four elite bread wheat cultivars from Western Australia: Bonnie Rock, IGW-2971, Magenta and Wyalkatchem, with the aim of evaluating transgene performance in different genetic backgrounds. The selected pollinator line, BW8-9-10-3, contained multiple transgene copies, had significantly improved drought tolerance compared with wild-type plants and showed no growth and development penalties or abnormalities. A single hybrid plant was selected from each cross-combination for three rounds of backcrossing with the corresponding maternal wheat cultivar. The transgene was detected in all four F1 BC3 combinations, but stress-inducible transgene expression was found in only three of the four combinations. Under well-watered conditions, the phenotypes and grain yield components of the F2 BC3 transgene-expressing lines were similar to those of corresponding recurrent parents and null-segregants. Under severe drought conditions, the backcross lines demonstrated 12-18% higher survival rates than the corresponding control plants. Two from four F3 BC3 transgenic lines showed significantly higher yield (18.9% and 21.5%) than control plants under limited water conditions. There was no induction of transgene expression under cold stress, and therefore, no improvement of frost tolerance observed in the progenies of drought-tolerant F3 BC3 lines. | - |
dc.description.statementofresponsibility | Yuri Shavrukov, Manahil Baho, Sergiy Lopato and Peter Langridge | - |
dc.language.iso | en | - |
dc.publisher | Wiley | - |
dc.rights | © 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd | - |
dc.source.uri | http://dx.doi.org/10.1111/pbi.12385 | - |
dc.subject | DREB transcription factor | - |
dc.subject | backcrossing | - |
dc.subject | drought | - |
dc.subject | frost | - |
dc.subject | hybrids | - |
dc.subject | transgenic wheat | - |
dc.title | The TaDREB3 transgene transferred by conventional crossings to different genetic backgrounds of bread wheat improves drought tolerance | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1111/pbi.12385 | - |
dc.relation.grant | ARC | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Langridge, P. [0000-0001-9494-400X] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 7 |
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