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Type: Journal article
Title: Identification of proteins associated with malting quality in a subset of wild barley introgression lines
Author: March, T.
Richter, D.
Colby, T.
Harzen, A.
Schmidt, J.
Pillen, K.
Citation: Proteomics, 2012; 12(18):2843-2851
Publisher: Wiley-VCH Verlag
Issue Date: 2012
ISSN: 1615-9853
Statement of
Timothy J. March, Daniela Richter, Thomas Colby, Anne Harzen, Jürgen Schmidt, and Klaus Pillen
Abstract: Malted barley is an important ingredient used in the brewing and distilling industry worldwide. In this study, we used a proteomics approach to investigate the biochemical function of previously identified quantitative trait loci (QTLs) on barley chromosomes 1H and 4H that influence malting quality. Using a subset of barley introgression lines containing wild barley (Hordeum vulgare ssp. spontaneum) alleles at these QTLs, we validated that wild barley alleles at the chromosome 1H QTL reduced overall malting quality, whereas wild barley alleles at the chromosome 4H QTL improved the malting quality parameters α-amylase activity, VZ45, and Kolbach index compared to the control genotype Scarlett. 2DE was used to detect changes in protein expression during the first 72 h of micromalting associated with these QTLs. In total, 16 protein spots showed a significant change in expression between the introgression lines and Scarlett, of which 14 were successfully identified with MS. Notably, the wild barley alleles in the line containing the chromosome 4H QTL showed a sixfold increased expression of a limit dextrinase inhibitor. The possible role of the identified proteins in malting quality is discussed. The knowledge gained will assist ongoing research toward cloning the genes underlying these important QTL.
Keywords: 2D protein electrophoresis; Barley introgression lines; Malting quality; Mass spectrometry; Plant proteomics
Rights: 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
DOI: 10.1002/pmic.201200117
Appears in Collections:Agriculture, Food and Wine publications
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