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Type: Journal article
Title: Over expression of rice chitinase gene in transgenic peanut (Arachis hypogaea L.) improves resistance against leaf spot
Author: Iqbal, M.
Nazir, F.
Ali, S.
Asif, M.
Zafar, Y.
Iqbal, J.
Ali, G.
Citation: Molecular Biotechnology, 2012; 50(2):129-136
Publisher: Humana Press
Issue Date: 2012
ISSN: 1073-6085
Statement of
Muhammad Munir Iqbal, Farhat Nazir, Shaukat Ali, M. Ahsan Asif, Yusuf Zafar, Javaid Iqbal, Ghulam Muhammad Ali
Abstract: A Rice chitinase-3 under enhance version of CaMV 35S was introduced into peanut (Arachis hypogaea L.) through Agrobacterium mediation. Agrobacterium tumefaciens strain LB4404 was used harboring the binary vector (pB1333-EN4-RCG3) containing the chitinase (chit) and hygromycin resistance (hpt) gene as selectable marker. Putative transgenic shoots were regenerated and grown on MS medium supplemented with 5 mg/l BAP, 1 mg/l kinetin, and 30 mg/l hygromycin. Elongated shoots were examined for the presence of the integrated rice chitinase gene along with hygromycin gene as selectable. The integration pattern of transgene in the nuclear genome of the putative transformed plants (T(0)) was confirmed through Southern hybridization analysis of the genomic DNA. Survival rate of the in vitro regenerated plantlets was over 60% while healthy putatively transgenic (T(0)) plants with over 42% transformation frequency were produced through Agrobacterium mediated gene transfer of the rice chitinase gene and all the plants flowered and set seed normally. T1 plants were tested for resistance against Cercospora arachidicola by infection with the microspores. Transgenic strains exhibited a higher resistance than the control (non-transgenic plants). chitinase gene expression in highly resistant transgenic strains was compared to that of a susceptible control. A good correlation was observed between chitinase activity and fungal pathogen resistance.
Keywords: Agrobacterium tumefaciens
Arachis hypogaea
Rice chitinase gene
Rights: © Springer Science+Business Media, LLC 2011
DOI: 10.1007/s12033-011-9426-2
Appears in Collections:Agriculture, Food and Wine publications
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