Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/9250
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Type: Journal article
Title: Increased production of TGF-β and apoptosis of T lymphocytes isolated from peripheral blood in COPD
Other Titles: Increased production of TGF-beta and apoptosis of T lymphocytes isolated from peripheral blood in COPD
Author: Hodge, S.
Hodge, G.
Reynolds, P.
Scicchitano, R.
Holmes, M.
Citation: American Journal of Physiology: Lung Cellular and Molecular Physiology, 2003; 285(2):L492-L499
Publisher: American Physiological Society
Issue Date: 2003
ISSN: 1040-0605
1522-1504
Statement of
Responsibility: 
S. J. Hodge , G. L. Hodge , P. N. Reynolds , R. Scicchitano , M. Holmes
Abstract: Chronic obstructive pulmonary disease (COPD) is associated with inflammation of airway epithelium, including an increase in the number of intraepithelial T cells. Increased apoptosis of these T cells has been reported in the airways in COPD, and although this process is critical for clearing excess activated T cells, excessive rates of apoptosis may result in unbalanced cellular homeostasis, defective clearance of apoptotic material by monocytes/macrophages, secondary necrosis, and prolongation of the inflammatory response. Lymphocytes are known to traffic between the airway and the peripheral circulation, thus we hypothesized that in COPD, circulating T cells may show an increased propensity to undergo apoptosis. We analyzed phytohemagglutinin (PHA)-stimulated peripheral blood T cells from COPD patients and controls for apoptosis using flow cytometry and staining with annexin V and 7-aminoactinomycin D. As several pathways are involved in induction of apoptosis of T cells, including transforming growth factor (TGF)-β/TGF receptor (TGFR), TNF-α/TNFR1, and Fas/Fas ligand, these mediators were also investigated in peripheral blood samples from these subject groups. Significantly increased apoptosis of PHA-stimulated T cells was observed in COPD (annexin positive 75.0 ± 14.7% SD vs. control 50.2 ± 21.8% SD, P = 0.006), along with upregulation of TNF-α/TNFR1, Fas, and TGFR. Monocyte production of TGF-β was also increased. In conclusion we have demonstrated the novel finding of increased apoptosis of stimulated T cells in COPD and have also shown that the increased T-cell death may be associated with upregulation of apoptotic pathways, TGF-β, TNF-α, and Fas in the peripheral blood in COPD.
Keywords: T-Lymphocytes
Humans
Pulmonary Disease, Chronic Obstructive
Transforming Growth Factor beta
Tumor Necrosis Factor-alpha
Vital Capacity
Forced Expiratory Volume
Lymphocyte Activation
Smoking
Apoptosis
Reference Values
Adult
Aged
Female
Male
Rights: Copyright © 2003 the American Physiological Society
DOI: 10.1152/ajplung.00428.2002
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