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https://hdl.handle.net/2440/9250
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dc.contributor.author | Hodge, S. | - |
dc.contributor.author | Hodge, G. | - |
dc.contributor.author | Reynolds, P. | - |
dc.contributor.author | Scicchitano, R. | - |
dc.contributor.author | Holmes, M. | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | American Journal of Physiology: Lung Cellular and Molecular Physiology, 2003; 285(2):L492-L499 | - |
dc.identifier.issn | 1040-0605 | - |
dc.identifier.issn | 1522-1504 | - |
dc.identifier.uri | http://hdl.handle.net/2440/9250 | - |
dc.description.abstract | Chronic obstructive pulmonary disease (COPD) is associated with inflammation of airway epithelium, including an increase in the number of intraepithelial T cells. Increased apoptosis of these T cells has been reported in the airways in COPD, and although this process is critical for clearing excess activated T cells, excessive rates of apoptosis may result in unbalanced cellular homeostasis, defective clearance of apoptotic material by monocytes/macrophages, secondary necrosis, and prolongation of the inflammatory response. Lymphocytes are known to traffic between the airway and the peripheral circulation, thus we hypothesized that in COPD, circulating T cells may show an increased propensity to undergo apoptosis. We analyzed phytohemagglutinin (PHA)-stimulated peripheral blood T cells from COPD patients and controls for apoptosis using flow cytometry and staining with annexin V and 7-aminoactinomycin D. As several pathways are involved in induction of apoptosis of T cells, including transforming growth factor (TGF)-β/TGF receptor (TGFR), TNF-α/TNFR1, and Fas/Fas ligand, these mediators were also investigated in peripheral blood samples from these subject groups. Significantly increased apoptosis of PHA-stimulated T cells was observed in COPD (annexin positive 75.0 ± 14.7% SD vs. control 50.2 ± 21.8% SD, P = 0.006), along with upregulation of TNF-α/TNFR1, Fas, and TGFR. Monocyte production of TGF-β was also increased. In conclusion we have demonstrated the novel finding of increased apoptosis of stimulated T cells in COPD and have also shown that the increased T-cell death may be associated with upregulation of apoptotic pathways, TGF-β, TNF-α, and Fas in the peripheral blood in COPD. | - |
dc.description.statementofresponsibility | S. J. Hodge , G. L. Hodge , P. N. Reynolds , R. Scicchitano , M. Holmes | - |
dc.language.iso | en | - |
dc.publisher | American Physiological Society | - |
dc.rights | Copyright © 2003 the American Physiological Society | - |
dc.source.uri | http://dx.doi.org/10.1152/ajplung.00428.2002 | - |
dc.subject | T-Lymphocytes | - |
dc.subject | Humans | - |
dc.subject | Pulmonary Disease, Chronic Obstructive | - |
dc.subject | Transforming Growth Factor beta | - |
dc.subject | Tumor Necrosis Factor-alpha | - |
dc.subject | Vital Capacity | - |
dc.subject | Forced Expiratory Volume | - |
dc.subject | Lymphocyte Activation | - |
dc.subject | Smoking | - |
dc.subject | Apoptosis | - |
dc.subject | Reference Values | - |
dc.subject | Adult | - |
dc.subject | Aged | - |
dc.subject | Female | - |
dc.subject | Male | - |
dc.title | Increased production of TGF-β and apoptosis of T lymphocytes isolated from peripheral blood in COPD | - |
dc.title.alternative | Increased production of TGF-beta and apoptosis of T lymphocytes isolated from peripheral blood in COPD | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1152/ajplung.00428.2002 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Hodge, S. [0000-0002-3602-9927] [0000-0002-9401-298X] | - |
dc.identifier.orcid | Reynolds, P. [0000-0002-2273-1774] | - |
Appears in Collections: | Aurora harvest 4 Medicine publications |
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