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Type: Journal article
Title: Expression and localization of homeodomain proteins DLX4, HB9 and HB24 in malignant and benign human colorectal tissues
Author: Hollington, P.
Neufing, P.
Kallionis, B.
Waring, P.
Bentel, J.
Wattchow, D.
Tilley, W.
Citation: Anticancer Research: international journal of cancer research and treatment, 2004; 24(2B):955-962
Publisher: Int Inst Anticancer Research
Issue Date: 2004
ISSN: 0250-7005
Statement of
Hollington, Paul ; Neufing, Petra ; Kalionis, Bill ; Waring, Paul ; Bentel, Jacky ; Wattchow, David ; Tilley, Wayne D
Abstract: <h4>Background</h4>The purpose of this study was to identify homeobox genes expressed in the human colon and to determine whether their expression levels were altered between matched non-malignant and malignant colon tissues.<h4>Materials and methods</h4>Homeobox genes expressed in colon tissue were identified by reverse transcription polymerase chain reaction (RT-PCR). Antibodies were raised to the homeodomain proteins DLX4, HB9 and HB24 and immunohistochemistry was performed on 3 moderately-differentiated tumors and their corresponding non-malignant colon tissue samples.<h4>Results</h4>The RT-PCR screen identified expression of DLX4, HB9, HB24 and MSX2 in the normal colon. Immunoaffinity purified polyclonal antisera raised against DLX4, HB9 and HB24 detect specific immunoreactivity in glandular epithelial cells, stromal cells of the lamina propria but not in the submucosa. Nuclear epithelial immunoreactivity of all three antibodies decreased in moderately-differentiated tumors compared to the corresponding matched non-malignant mucosa. These data suggest that differential expression of HB9, HB24 and DLX4 may be associated with colorectal carcinogenesis.
Keywords: Homeobox gene-expression
Drosophila midgut morphogenesis
Beta-globin gene
Homeotic genes
Sacral agenesis
Transgenic mice
CDX2 homeobox
Rights: © 2004 International Institute of Anticancer Research
Appears in Collections:Aurora harvest
Medicine publications

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