Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/92746
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Type: Journal article
Title: AdcA and AdcAII employ distinct zinc acquisition mechanisms and contribute additively to zinc homeostasis in Streptococcus pneumoniae
Author: Plumptre, C.
Eijkelkamp, B.
Morey, J.
Behr, F.
Couñago, R.
Ogunniyi, A.
Kobe, B.
O'Mara, M.
Paton, J.
McDevitt, C.
Citation: Molecular Microbiology, 2014; 91(4):834-851
Publisher: Wiley
Issue Date: 2014
ISSN: 1365-2958
0950-382X
Statement of
Responsibility: 
Charles D. Plumptre, Bart A. Eijkelkamp, Jacqueline R. Morey, Felix Behr, Rafael M. Couñago, Abiodun D. Ogunniyi, Bostjan Kobe, Megan L. O, Mara, James C. Paton, and Christopher A. McDevitt
Abstract: Streptococcus pneumoniae is a globally significant human pathogen responsible for nearly 1 million deaths annually. Central to the ability of S. pneumoniae to colonize and mediate disease in humans is the acquisition of zinc from the host environment. Zinc uptake in S. pneumoniae occurs via the ATP-binding cassette transporter AdcCB, and, unusually, two zinc-binding proteins, AdcA and AdcAII. Studies have suggested that these two proteins are functionally redundant, although AdcA has remained uncharacterized by biochemical methods. Here we show that AdcA is a zinc-specific substrate-binding protein (SBP). By contrast with other zinc-binding SBPs, AdcA has two zinc-binding domains: a canonical amino-terminal cluster A-I zinc-binding domain and a carboxy-terminal zinc-binding domain, which has homology to the zinc-chaperone ZinT from Gram-negative organisms. Intriguingly, this latter feature is absent from AdcAII and suggests that the two zinc-binding SBPs of S. pneumoniae employ different modalities in zinc recruitment. We further show that AdcAII is reliant upon the polyhistidine triad proteins for zinc in vitro and in vivo. Collectively, our studies suggest that, despite the overlapping roles of the two SBPs in zinc acquisition, they may have unique mechanisms in zinc homeostasis and act in a complementary manner during host colonization.
Keywords: Streptococcus pneumoniae; Zinc; ATP-Binding Cassette Transporters; Bacterial Proteins; Homeostasis; Protein Structure, Tertiary; Protein Binding
Rights: © 2013 John Wiley & Sons Ltd
RMID: 0030008789
DOI: 10.1111/mmi.12504
Grant ID: http://purl.org/au-research/grants/arc/DP120103957
http://purl.org/au-research/grants/nhmrc/1022240
http://purl.org/au-research/grants/nhmrc/565526
Appears in Collections:Molecular and Biomedical Science publications

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