Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/92761
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Type: Journal article
Title: KCNT1 gain of function in 2 epilepsy phenotypes is reversed by quinidine
Author: Milligan, C.
Li, M.
Gazina, E.
Heron, S.
Nair, U.
Trager, C.
Reid, C.
Venkat, A.
Younkin, D.
Dlugos, D.
Petrovski, S.
Goldstein, D.
Dibbens, L.
Scheffer, I.
Berkovic, S.
Petrou, S.
Citation: Annals of Neurology, 2014; 75(4):581-590
Publisher: Wiley
Issue Date: 2014
ISSN: 0364-5134
1531-8249
Statement of
Responsibility: 
Carol J. Milligan, Melody Li, Elena V. Gazina, Sarah E. Heron, Umesh Nair, Chantel Trager, Christopher A. Reid, Anu Venkat, Donald P. Younkin, Dennis J. Dlugos, Slavé Petrovski, David B. Goldstein, Leanne M. Dibbens, Ingrid E. Scheffer, Samuel F. Berkovic, and Steven Petrou
Abstract: OBJECTIVE: Mutations in KCNT1 have been implicated in autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE) and epilepsy of infancy with migrating focal seizures (EIMFS). More recently, a whole exome sequencing study of epileptic encephalopathies identified an additional de novo mutation in 1 proband with EIMFS. We aim to investigate the electrophysiological and pharmacological characteristics of hKCNT1 mutations and examine developmental expression levels. METHODS: Here we use a Xenopus laevis oocyte-based automated 2-electrode voltage clamp assay. The effects of quinidine (100 and 300 μM) are also tested. Using quantitative reverse transcriptase polymerase chain reaction, the relative levels of mouse brain mKcnt1 mRNA expression are determined. RESULTS: We demonstrate that KCNT1 mutations implicated in epilepsy cause a marked increase in function. Importantly, there is a significant group difference in gain of function between mutations associated with ADNFLE and EIMFS. Finally, exposure to quinidine significantly reduces this gain of function for all mutations studied. INTERPRETATION: These results establish direction for a targeted therapy and potentially exemplify a translational paradigm for in vitro studies informing novel therapies in a neuropsychiatric disease.
Keywords: Mice, Inbred C57BL
Oocytes
Tetradecanoylphorbol Acetate
Quinidine
Nerve Tissue Proteins
Potassium Channels
Patch-Clamp Techniques
Microinjections
Electric Stimulation
Dose-Response Relationship, Drug
Mutation
Xenopus laevis
Membrane Potentials
Time Factors
Voltage-Gated Sodium Channel Blockers
Rights: © 2014 American Neurological Association
DOI: 10.1002/ana.24128
Grant ID: http://purl.org/au-research/grants/nhmrc/628952
http://purl.org/au-research/grants/nhmrc/1016715
http://purl.org/au-research/grants/nhmrc/1005050
http://purl.org/au-research/grants/nhmrc/466671
http://purl.org/au-research/grants/nhmrc/1006110
http://purl.org/au-research/grants/nhmrc/1032603
Appears in Collections:Aurora harvest 7
Paediatrics publications

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