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Type: Thesis
Title: PSC1: a protein with multiple roles in RNA metabolism.
Author: Davey, Philippa Lois
Issue Date: 2014
School/Discipline: School of Molecular and Biomedical Science
Abstract: Peri-implantation stem cell 1 (Psc1) is a developmentally regulated protein that is down regulated as cells of the blastocyst inner cell mass differentiate into primitive ectoderm in vivo, and as embryonic stem (ES) cells differentiate in vitro. The function of Psc1 is unknown, however the Psc1 protein sequence contains multiple domains that suggest a role in RNA metabolism. These include an RNA recognition motif (RRM) that has been shown to bind RNA in vitro, and a motif known as an RS domain that contains multiple alternating serine and arginine dipeptide repeats, and is found in many proteins involved in RNA processing. Psc1 localises with RNA metabolism proteins and to sites in the nucleus known as nuclear or splicing speckles, as well as to unidentified speckles in the cytoplasm. The work in this thesis has shown that multiple alternative forms of Psc1 exist at both the RNA and protein level, and that regulation of these alternative forms of Psc1 occurs during ES cell differentiation. Further evidence for a role in RNA metabolism and the identification of Psc1 function has been advanced through the identification of Psc1 protein binding partners Sart1 and Pabpn1. These results have lead to a model where Psc1 functions at multiple stages during the maturation of specific RNA transcripts, potentially during splicing and spliceosome formation though an interaction with Sart1, and during mRNA nuclear export through an interaction with Pabpn1.
Advisor: Rathjen, Peter David
Dissertation Note: Thesis (Ph.D.) -- University of Adelaide, School of Molecular and Biomedical Science, 2014
Keywords: SR related protein; RNA metabolism; nuclear speckles; cytospeckle; ES cell
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