Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/92903
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Type: Journal article
Title: Development and use of a quantum dot probe to track multiple yeast strains in mixed culture
Author: Gustafsson, F.
Whiteside, M.
Jiranek, V.
Durall, D.
Citation: Scientific Reports, 2014; 4(1):6971-1-6971-6
Publisher: Nature Publishing Group
Issue Date: 2014
ISSN: 2045-2322
2045-2322
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Responsibility: 
Frida S. Gustafsson, Matthew D. Whiteside, Vladimir Jiranek and Daniel M. Durall
Abstract: Saccharomyces cerevisiae strains vary in their ability to develop and enhance sensory attributes of alcoholic beverages and are often found growing in mixed strain fermentations; however, quantifying individual strains is challenging due to quantification inaccuracies, low marker longevity, and compromised kinetics. We developed a fluorescent probe, consisting of glutathione molecules conjugated to a quantum dot (QD). Two S. cerevisiae strains were incubated with different coloured probes (QD attached to glutathione molecules, QD-GSH), fermented at multiple ratios, and quantified using confocal microscopy. The QD method was compared with a culture method using microsatellite DNA analysis (MS method). Probes were taken up by an ADP1 encoded transporter, transferred from mother cell to daughter cell, detectable in strains throughout fermentation, and were non-toxic. This resulted in a new quantification method that was more accurate and efficient than the MS method.
Keywords: Quantum dots; Molecular biology
Description: Published 10 November 2014
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
RMID: 0030014998
DOI: 10.1038/srep06971
Appears in Collections:Agriculture, Food and Wine publications

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