Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Evidence that phospholipids play a key role in pre-β apoA-I formation and high-density lipoprotein remodeling
Other Titles: Evidence that phospholipids play a key role in pre-beta apoA-I formation and high-density lipoprotein remodeling
Author: Rye, K.
Duong, M.
Psaltis, M.
Curtiss, L.
Bonnet, D.
Stocker, R.
Barter, P.
Citation: Biochemistry, 2002; 41(41):12538-12545
Publisher: Amer Chemical Soc
Issue Date: 2002
ISSN: 0006-2960
Statement of
Kerry-Anne Rye, MyNgan Duong, Maria K. Psaltis, Linda K. Curtiss, David J. Bonnet, Roland Stocker, and Philip J. Barter
Abstract: The initial plasma acceptor of unesterified cholesterol and phospholipids from peripheral cells has been identified as pre-beta migrating, lipid-free, or lipid-poor apolipoprotein (apo) A-I (pre-beta apoA-I). Pre-beta apoA-I is formed when plasma factors, such as cholesteryl ester transfer protein (CETP), remodel high-density lipoproteins (HDL). The aim of this study is to determine how phospholipids influence pre-beta apoA-I formation during the CETP-mediated remodeling of HDL. Reconstituted HDL (rHDL) containing either 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC), 1-palmitoyl-2-linoleoyl phosphatidylcholine (PLPC), 1-palmitoyl-2-arachidonyl phosphatidylcholine (PAPC), or 1-palmitoyl-2-docosahexanoyl phosphatidylcholine (PDPC) as the only phospholipid were prepared. The rHDL were comparable in size and core lipid/protein molar ratio and contained only cholesteryl esters in their core and apoA-I as the sole apolipoprotein. The (POPC)rHDL, (PLPC)rHDL, (PAPC)rHDL, and (PDPC)rHDL were respectively incubated for 0-24 h with CETP and microemulsions containing triolein and either POPC, PLPC, PAPC, or PDPC. The rate at which the rHDL were depleted of core lipids and remodeled to small particles varied widely with (POPC)rHDL < (PLPC)rHDL < (PDPC)rHDL approximately (PAPC)rHDL. Pre-beta apoA-I was not formed in the (POPC)rHDL incubations. Pre-beta apoA-I was apparent by 24 h in the (PLPC)rHDL incubations and by 12 h in the (PAPC)rHDL and (PDPC)rHDL incubations. The enhanced formation of pre-beta apoA-I in the (PAPC)rHDL and (PDPC)rHDL incubations reflected the increased core lipid depletion of the particles combined with the destabilization and progressive exclusion of apoA-I from the particle surface. In conclusion, these results show that phospholipids play a key role in the CETP-mediated remodeling of rHDL and pre-beta apoA-I formation.
Keywords: Humans
Phospholipid Ethers
Cholesterol Esters
Lipoproteins, HDL
Apolipoprotein A-I
Carrier Proteins
Electrophoresis, Polyacrylamide Gel
Surface Plasmon Resonance
Cholesterol Ester Transfer Proteins
High-Density Lipoproteins, Pre-beta
DOI: 10.1021/bi025998k
Appears in Collections:Aurora harvest 4
Medicine publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.