Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/93016
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Type: Journal article
Title: Major haplotype divergence including multiple germin-like protein genes, at the wheat Sr2 adult plant stem rust resistance locus
Author: Mago, R.
Tabe, L.
Vautrin, S.
Simkova, H.
Kubalakova, M.
Upadhyaya, N.
Berges, H.
Kong, X.
Breen, J.
Dolezel, J.
Appels, R.
Ellis, J.
Spielmeyer, W.
Citation: BMC Plant Biology, 2014; 14(1):379-1-379-11
Publisher: BioMed Central
Issue Date: 2014
ISSN: 1471-2229
1471-2229
Statement of
Responsibility: 
Rohit Mago, Linda Tabe, Sonia Vautrin, Hana Šimková, Marie Kubaláková, Narayana Upadhyaya, Hélène Berges, Xiuying Kong, James Breen, Jaroslav Doležel, Rudi Appels, Jeffrey G Ellis and Wolfgang Spielmeyer
Abstract: BACKGROUND: The adult plant stem rust resistance gene Sr2 was introgressed into hexaploid wheat cultivar (cv) Marquis from tetraploid emmer wheat cv Yaroslav, to generate stem rust resistant cv Hope in the 1920s. Subsequently, Sr2 has been widely deployed and has provided durable partial resistance to all known races of Puccinia graminis f. sp. tritici. This report describes the physical map of the Sr2-carrying region on the short arm of chromosome 3B of cv Hope and compares the Hope haplotype with non-Sr2 wheat cv Chinese Spring. RESULTS: Sr2 was located to a region of 867 kb on chromosome 3B in Hope, which corresponded to a region of 567 kb in Chinese Spring. The Hope Sr2 region carried 34 putative genes but only 17 were annotated in the comparable region of Chinese Spring. The two haplotypes differed by extensive DNA sequence polymorphisms between flanking markers as well as by a major insertion/deletion event including ten Germin-Like Protein (GLP) genes in Hope that were absent in Chinese Spring. Haplotype analysis of a limited number of wheat genotypes of interest showed that all wheat genotypes carrying Sr2 possessed the GLP cluster; while, of those lacking Sr2, some, including Marquis, possessed the cluster, while some lacked it. Thus, this region represents a common presence-absence polymorphism in wheat, with presence of the cluster not correlated with presence of Sr2. Comparison of Hope and Marquis GLP genes on 3BS found no polymorphisms in the coding regions of the ten genes but several SNPs in the shared promoter of one divergently transcribed GLP gene pair and a single SNP downstream of the transcribed region of a second GLP. CONCLUSION: Physical mapping and sequence comparison showed major haplotype divergence at the Sr2 locus between Hope and Chinese Spring. Candidate genes within the Sr2 region of Hope are being evaluated for the ability to confer stem rust resistance. Based on the detailed mapping and sequencing of the locus, we predict that Sr2 does not belong to the NB-LRR gene family and is not related to previously cloned, race non-specific rust resistance genes Lr34 and Yr36.
Keywords: Adult plant resistance (APR); Map-based cloning; Sr2 ; Germin-like proteins (GLPs); Wheat stem rust; Puccinia graminis ; Physical mapping; Gene expression
Description: Published: 30 December 2014
Rights: © 2014 Mago et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
RMID: 0030017748
DOI: 10.1186/s12870-014-0379-z
Appears in Collections:Agriculture, Food and Wine publications

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