Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/93017
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Distribution, structure and biosynthetic gene families of (1,3;1,4)-β-glucan in Sorghum bicolor
Other Titles: Distribution, structure and biosynthetic gene families of (1,3;1,4)-beta-glucan in Sorghum bicolor
Author: Ermawar, R.
Collins, H.
Byrt, C.
Betts, N.
Henderson, M.
Shirley, N.
Schwerdt, J.
Lahnstein, J.
Fincher, G.
Burton, R.
Citation: Journal of Integrative Plant Biology, 2015; 57(4):429-445
Publisher: Wiley
Issue Date: 2015
ISSN: 1672-9072
1744-7909
Statement of
Responsibility: 
Riksfardini A. Ermawar, Helen M. Collins, Caitlin S. Byrt, Natalie S. Betts, Marilyn Henderson, Neil J. Shirley, Julian Schwerdt, Jelle Lahnstein, Geoffrey B. Fincher and Rachel A. Burton
Abstract: In cereals, the presence of soluble polysaccharides including (1,3;1,4)-β-glucan has downstream implications for human health, animal feed and biofuel applications. Sorghum bicolor (L.) Moench is a versatile crop, but there are limited reports regarding the content of such soluble polysaccharides. Here, the amount of (1,3;1,4)-β-glucan present in sorghum tissues was measured using a Megazyme assay. Very low amounts were present in the grain, ranging from 0.16%-0.27% (w/w), while there was a greater quantity in vegetative tissues at 0.12-1.71% (w/w). The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1. The distribution of (1,3;1,4)-β-glucan was examined using a specific antibody and observed with fluorescence and transmission electron microscopy. Micrographs showed a variable distribution of (1,3;1,4)-β-glucan influenced by temporal and spatial factors. The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families were defined. Transcript profiling of these genes across sorghum tissues was carried out using real-time quantitative polymerase chain reaction, indicating that, as in other cereals, CslF6 transcripts dominated.
Keywords: Cellulose synthase-like genes; cell wall composition; grain; mixed-linkage glucan; sorghum
Description: Article first published online: 31 MAR 2015
Rights: © 2015 Institute of Botany, Chinese Academy of Sciences
RMID: 0030022648
DOI: 10.1111/jipb.12338
Appears in Collections:Agriculture, Food and Wine publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.