Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/9313
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Type: Journal article
Title: Structure of the activation domain of the GM-CSF/IL-3/IL-5 receptor common b-chain bound to an antagonist
Author: Rossjohn, J.
McKinstry, W.
Woodcock, J.
Mc Clure, B.
Hercus, T.
Parker, M.
Lopez, A.
Bagley, C.
Citation: Blood, 2000; 95(8):2491-2498
Publisher: Amer Soc Hematology
Issue Date: 2000
ISSN: 0006-4971
1528-0020
Statement of
Responsibility: 
Jamie Rossjohn, William J. McKinstry, Joanna M. Woodcock, Barbara J. McClure, Timothy R. Hercus, Michael W. Parker, Angel F. Lopez, and Christopher J. Bagley
Abstract: Heterodimeric cytokine receptors generally consist of a major cytokine-binding subunit and a signaling subunit. The latter can transduce signals by more than 1 cytokine, as exemplified by the granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-2 (IL-2), and IL-6 receptor systems. However, often the signaling subunits in isolation are unable to bind cytokines, a fact that has made it more difficult to obtain structural definition of their ligand-binding sites. This report details the crystal structure of the ligand-binding domain of the GM-CSF/IL-3/IL-5 receptor beta-chain (beta(c)) signaling subunit in complex with the Fab fragment of the antagonistic monoclonal antibody, BION-1. This is the first single antagonist of all 3 known eosinophil-producing cytokines, and it is therefore capable of regulating eosinophil-related diseases such as asthma. The structure reveals a fibronectin type III domain, and the antagonist-binding site involves major contributions from the loop between the B and C strands and overlaps the cytokine-binding site. Furthermore, tyrosine(421) (Tyr(421)), a key residue involved in receptor activation, lies in the neighboring loop between the F and G strands, although it is not immediately adjacent to the cytokine-binding residues in the B-C loop. Interestingly, functional experiments using receptors mutated across these loops demonstrate that they are cooperatively involved in full receptor activation. The experiments, however, reveal subtle differences between the B-C loop and Tyr(421), which is suggestive of distinct functional roles. The elucidation of the structure of the ligand-binding domain of beta(c) also suggests how different cytokines recognize a single receptor subunit, which may have implications for homologous receptor systems. (Blood. 2000;95:2491-2498)
Keywords: Cell Line; Humans; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; Receptors, Interleukin-3; Receptors, Interleukin; Antibodies, Monoclonal; Ligands; Epitope Mapping; Binding Sites; Protein Conformation; Protein Binding; Molecular Sequence Data; Receptors, Interleukin-5
RMID: 0001001596
DOI: 10.1182/blood.v95.8.2491
Appears in Collections:Medicine publications

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