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Type: Journal article
Title: Identification and validation of candidate Myb target genes
Author: Bartley, P.
Lutwyche, J.
Gonda, T.
Citation: Blood Cells Molecules and Diseases, 2001; 27(2):409-415
Publisher: Academic Press Inc
Issue Date: 2001
ISSN: 1079-9796
Statement of
Paul A. Bartley, Jodi K. Lutwyche and Thomas J. Gonda
Abstract: While a considerable number of candidate Myb target genes have been reported to date, most of these are likely to play little or no role in transformation by myb oncogenes. Here we have used a conditionally myb-transformed myeloid cell line (ERMYB) to further examine Myb regulation of one candidate target gene--c-myc--that has the potential to affect cell proliferation. It was found that the major influence on c-myc expression was the presence of cytokine (GM-CSF) rather than Myb activity. We also describe the application of PCR-based subtractive hybridization and low-density cDNA array screening, in conjunction with the ERMYB line, to the identification of additional Myb target genes. Preliminary identification of a number of candidates is reported; these include myeloperoxidase, which is known to have essential Myb-binding sites in its regulatory region.
Keywords: Cell Line, Transformed; Humans; Cell Transformation, Neoplastic; Gene Targeting; Gene Expression Regulation, Neoplastic; Genes, myb; Genes, myc
Description: Copyright © 2001 Academic Press. All rights reserved.
RMID: 0020011011
DOI: 10.1006/bcmd.2001.0398
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Appears in Collections:Medicine publications

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