Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/9314
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Identification and validation of candidate Myb target genes |
Author: | Bartley, P. Lutwyche, J. Gonda, T. |
Citation: | Blood Cells, Molecules and Diseases, 2001; 27(2):409-415 |
Publisher: | Academic Press Inc |
Issue Date: | 2001 |
ISSN: | 1079-9796 1096-0961 |
Statement of Responsibility: | Paul A. Bartley, Jodi K. Lutwyche and Thomas J. Gonda |
Abstract: | While a considerable number of candidate Myb target genes have been reported to date, most of these are likely to play little or no role in transformation by myb oncogenes. Here we have used a conditionally myb-transformed myeloid cell line (ERMYB) to further examine Myb regulation of one candidate target gene--c-myc--that has the potential to affect cell proliferation. It was found that the major influence on c-myc expression was the presence of cytokine (GM-CSF) rather than Myb activity. We also describe the application of PCR-based subtractive hybridization and low-density cDNA array screening, in conjunction with the ERMYB line, to the identification of additional Myb target genes. Preliminary identification of a number of candidates is reported; these include myeloperoxidase, which is known to have essential Myb-binding sites in its regulatory region. |
Keywords: | Cell Line, Transformed Humans Cell Transformation, Neoplastic Gene Targeting Gene Expression Regulation, Neoplastic Genes, myb Genes, myc |
Description: | Copyright © 2001 Academic Press. All rights reserved. |
DOI: | 10.1006/bcmd.2001.0398 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/622796/description#description |
Published version: | http://dx.doi.org/10.1006/bcmd.2001.0398 |
Appears in Collections: | Aurora harvest 4 Medicine publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.