Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/9328
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Type: Journal article
Title: Combination of stem cell factor and granulocyte colony-stimulating factor mobilizes the highest number of primitive haemopoietic progenitors as shown by pre-colony-forming unit (pre-CFU) assay
Author: Horsfall, M.
Hui, C.
To, L.
Begley, C.
Basser, R.
Simmons, P.
Citation: British Journal of Haematology, 2000; 109(4):751-758
Publisher: Blackwell Science Ltd
Issue Date: 2000
ISSN: 0007-1048
1365-2141
Statement of
Responsibility: 
M. J. Horsfall, C. H. Hui, L. B. To, C. G. Begley, R. L. Basser and P. J. Simmons
Abstract: Fifty-two patients with poor prognosis carcinoma of the breast underwent peripheral blood stem cell (PBSC) mobilization using five different regimens. The yields of primitive haemopoietic progenitors were quantified by a recently described pre-colony-forming unit (pre-CFU) assay using limiting dilution analysis (LDA). Results of days 14 and 35 pre-CFU were also correlated with conventional CD34+ cell enumeration, CFU-GM (granulocyte-macrophage) and long-term culture-initiating cell (LTCIC) assays. The yield of pre-CFUs with the combination of granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) was significantly higher than with G-CSF alone, cyclophosphamide (Cyclo) and granulocyte-monocyte colony-stimulating factor (GM-CSF), interleukin (IL)-3 and GM-CSF, or Cyclo alone. No significant correlation between neutrophil engraftment and pre-CFU could be demonstrated. Furthermore, CFU-GM was shown to bear a stronger correlation with pre-CFU and LTCIC than CD34+ cell measurement; thus, CFU-GM remains a useful biological tool for haemopoietic stem cell assay. We conclude that the combination of G-CSF and SCF mobilizes the highest number of pre-CFUs as measured by functional pre-CFU assay, which provides an alternative measurement of primitive haemopoietic progenitors to the LTCIC assay.
Keywords: pre-CFU assay; limiting dilution analysis; mobilization; stem cell factor
Provenance: Published Online: 24 Dec 2001
Rights: Copyright © 2009 Blackwell Publishing Ltd
RMID: 0001001261
DOI: 10.1046/j.1365-2141.2000.02108.x
Appears in Collections:Medicine publications

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