Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/93907
Type: Thesis
Title: Efficacy of laser and ultrasonic activated irrigation on eradicating a mixed species biofilm grown in the mesial roots of human mandibular molars.
Author: Race, Jonathan Peter
Issue Date: 2015
School/Discipline: School of Dentistry
Abstract: Aim To compare the efficacy of Er,Cr:YSGG laser and ultrasonic activated irrigation on eradicating a biofilm grown in the mesial roots of human mandibular molars. Methods A biofilm containing Enterococcus faecalis, Streptococcus sanguinis and Fusobacterium nucleatum was grown over 4 weeks in the mesial root canals of decoronated human mandibular molar teeth. Following removal from the flow cell, control roots (n=5) received no further treatment. The remaining tooth roots were chemomechanically prepared using different irrigating protocols: saline standard irrigation (Saline SI; n=15); 4% NaOCl and 15% EDTAC with ultrasonic activated irrigation (UAI; n=18); 4% NaOCl and 15% EDTAC with laser activated irrigation using power settings 0.5 W (LAI 0.5 W; n=18) or 0.75 W (LAI 0.75 W; n=10). Following treatment and crushing, bacteria were quantified by culturing (CFU/mL) and quantitative real-time PCR (qPCR). One tooth from each group was subjected to SEM analysis. Results Quantification by culturing revealed significant differences between controls and all other treatment groups. Significant differences were found between Saline SI and UAI, Saline SI and LAI 0.5 W and also between LAI 0.5 W and LAI 0.75 W. No significant differences were found between Saline SI and LAI 0.75 W or between UAI and LAI 0.5 W or LAI 0.75 W. From qPCR results, significant differences were found between controls and all other treatment groups. No statistically significant differences were found between Saline SI and UAI, LAI 0.5 W or LAI 0.75 W. Conclusions Both culture and molecular techniques showed that mechanical preparation significantly reduces bacteria from the root canals of lower molar mesial roots. Further reductions were achieved by irrigating with 4% NaOCl and 15% EDTAC UAI or 4% NaOCl and 15% EDTAC LAI. No significant reductions in bacterial number were found between UAI and LAI protocols.
Advisor: Cathro, Peter Robert
Zilm, Peter Stephen
Dissertation Note: Thesis (D.Clin.Dent.) -- University of Adelaide, School of Dentistry, 2015
Keywords: laser; ultrasonic; activate; irrigation; molar; biofilm; E. faecalis; S. sanguinins; F. nucleatum
Provenance: This electronic version is made publicly available by the University of Adelaide in accordance with its open access policy for student theses. Copyright in this thesis remains with the author. This thesis may incorporate third party material which has been used by the author pursuant to Fair Dealing exceptions. If you are the owner of any included third party copyright material you wish to be removed from this electronic version, please complete the take down form located at: http://www.adelaide.edu.au/legals
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