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Type: Journal article
Title: Comprehensive profiling analysis of actively resorbing osteoclasts identifies critical signaling pathways regulated by bone substrate
Author: Purdue, P.
Crotti, T.
Shen, Z.
Swantek, J.
Li, J.
Hill, J.
Hanidu, A.
Dimock, J.
Nabozny, G.
Goldring, S.
McHugh, K.
Citation: Scientific Reports, 2014; 4(1):7595-1-7595-11
Publisher: Nature
Issue Date: 2014
ISSN: 2045-2322
Statement of
P. Edward Purdue, Tania N. Crotti, Zhenxin Shen, Jennifer Swantek, Jun Li, Jonathan Hill, Adedayo Hanidu, Janice Dimock, Gerald Nabozny, Steven R. Goldring, Kevin P. McHugh
Abstract: As the only cells capable of efficiently resorbing bone, osteoclasts are central mediators of both normal bone remodeling and pathologies associates with excessive bone resorption. However, despite the clear evidence of interplay between osteoclasts and the bone surface in vivo, the role of the bone substrate in regulating osteoclast differentiation and activation at a molecular level has not been fully defined. Here, we present the first comprehensive expression profiles of osteoclasts differentiated on authentic resorbable bone substrates. This analysis has identified numerous critical pathways coordinately regulated by osteoclastogenic cytokines and bone substrate, including the transition from proliferation to differentiation, and sphingosine-1-phosphate signaling. Whilst, as expected, much of this program is dependent upon integrin beta 3, the pre-eminent mediator of osteoclast-bone interaction, a surprisingly significant portion of the bone substrate regulated expression signature is independent of this receptor. Together, these findings identify an important hitherto underappreciated role for bone substrate in osteoclastogenesis.
Keywords: Extracellular signalling molecules; transcriptions
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit
RMID: 0030022277
DOI: 10.1038/srep07595
Appears in Collections:Medicine publications

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