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Type: Journal article
Title: Cyclooxygenase inhibition improves endothelial vasomotor dysfunction of visceral adipose arterioles in human obesity
Author: Farb, M.
Tiwari, S.
Karki, S.
Ngo, D.
Carmine, B.
Hess, D.
Zuriaga, M.
Walsh, K.
Fetterman, J.
Hamburg, N.
Vita, J.
Apovian, C.
Gokce, N.
Citation: Obesity, 2014; 22(2):349-355
Publisher: The Obesity Society
Issue Date: 2014
ISSN: 1930-7381
Statement of
Melissa G. Farb, Stephanie Tiwari, Shakun Karki, Doan T.M. Ngo, Brian Carmine, Donald T. Hess, Maria A. Zuriaga, Kenneth Walsh, Jessica L. Fetterman, Naomi M. Hamburg, Joseph A. Vita, Caroline M. Apovian and Noyan Gokce
Abstract: OBJECTIVE: The purpose of this study was to determine whether cyclooxygenase inhibition improves vascular dysfunction of adipose microvessels from obese humans. DESIGN AND METHODS: In 20 obese subjects (age 37 ± 12 years, BMI 47 ± 8 kg/m²), subcutaneous and visceral fat were collected during bariatric surgery and characterized for adipose depot-specific gene expression, endothelial cell phenotype, and microvascular function. Vasomotor function was assessed in response to endothelium-dependent agonists using videomicroscopy of small arterioles from fat. RESULTS: Arterioles from visceral fat exhibited impaired endothelium-dependent, acetylcholine-mediated vasodilation, compared to the subcutaneous depot (P < 0.001). Expression of mRNA transcripts relevant to the cyclooxygenase pathway was upregulated in visceral compared to subcutaneous fat. Pharmacological inhibition of cyclooxygenase with indomethacin improved endothelium-dependent vasodilator function of arterioles from visceral fat by twofold (P = 0.01), whereas indomethacin had no effect in the subcutaneous depot. Indomethacin increased activation via serine-1177 phosphorylation of endothelial nitric oxide synthase in response to acetylcholine in endothelial cells from visceral fat. Inhibition of endothelial nitric oxide synthase with N(ω)-nitro-L-arginine methyl ester abrogated the effects of cyclooxygenase-inhibition suggesting that vascular actions of indomethacin were related to increased nitric oxide bioavailability. CONCLUSIONS: Our findings suggest that cyclooxygenase-mediated vasoconstrictor prostanoids partly contribute to endothelial dysfunction of visceral adipose arterioles in human obesity.
Keywords: Arterioles
Enzyme Inhibitors
Cyclooxygenase Inhibitors
Body Mass Index
Rights: © 2013 The Obesity Society
DOI: 10.1002/oby.20505
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