Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/94243
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Type: Journal article
Title: Whole genome capture of vector-borne pathogens from mixed DNA samples: a case study of Borrelia burgdorferi
Author: Carpi, G.
Walter, K.
Bent, S.
Hoen, A.
Diuk-Wasser, M.
Caccone, A.
Citation: BMC Genomics, 2015; 16(1):434-1-434-11
Publisher: BioMed Central
Issue Date: 2015
ISSN: 1471-2164
1471-2164
Statement of
Responsibility: 
Giovanna Carpi, Katharine S. Walter, Stephen J. Bent, Anne Gatewood Hoen, Maria Diuk-Wasser, and Adalgisa Caccone
Abstract: BACKGROUND: Rapid and accurate retrieval of whole genome sequences of human pathogens from disease vectors or animal reservoirs will enable fine-resolution studies of pathogen epidemiological and evolutionary dynamics. However, next generation sequencing technologies have not yet been fully harnessed for the study of vector-borne and zoonotic pathogens, due to the difficulty of obtaining high-quality pathogen sequence data directly from field specimens with a high ratio of host to pathogen DNA. RESULTS: We addressed this challenge by using custom probes for multiplexed hybrid capture to enrich for and sequence 30 Borrelia burgdorferi genomes from field samples of its arthropod vector. Hybrid capture enabled sequencing of nearly the complete genome (~99.5 %) of the Borrelia burgdorferi pathogen with 132-fold coverage, and identification of up to 12,291 single nucleotide polymorphisms per genome. CONCLUSIONS: The proprosed culture-independent method enables efficient whole genome capture and sequencing of pathogens directly from arthropod vectors, thus making population genomic study of vector-borne and zoonotic infectious diseases economically feasible and scalable. Furthermore, given the similarities of invertebrate field specimens to other mixed DNA templates characterized by a high ratio of host to pathogen DNA, we discuss the potential applicabilty of hybrid capture for genomic study across diverse study systems.
Keywords: Hybrid capture; whole-genome sequencing; SNPs; tick-borne pathogens; Lyme disease
Rights: © 2015 Carpi et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
DOI: 10.1186/s12864-015-1634-x
Appears in Collections:Aurora harvest 2
Molecular and Biomedical Science publications

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