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Type: Journal article
Title: A novel porcine model of traumatic thoracic spinal cord injury
Author: Lee, J.
Jones, C.
Okon, E.
Anderson, L.
Tigchelaar, S.
Kooner, P.
Godbey, T.
Chua, B.
Gray, G.
Hildebrandt, R.
Cripton, P.
Tetzlaff, W.
Kwon, B.
Citation: Journal of Neurotrauma, 2013; 30(3):142-159
Publisher: Mary Ann Liebert, Inc.
Issue Date: 2013
ISSN: 0897-7151
Statement of
Jae H. T. Lee, Claire F. Jones, Elena B. Okon, Lisa Anderson, Seth Tigchelaar, Paul Kooner, Tamara Godbey, Bev Chua, Gordon Gray, Rhonda Hildebrandt, Peter Cripton, Wolfram Tetzlaff, and Brian K. Kwon
Abstract: Spinal cord injury (SCI) researchers have predominately utilized rodents and mice for in vivo SCI modeling and experimentation. From these small animal models have come many insights into the biology of SCI, and a growing number of novel treatments that promote behavioral recovery. It has, however, been difficult to demonstrate the efficacy of such treatments in human clinical trials. A large animal SCI model that is an intermediary between rodent and human SCI may be a valuable translational research resource for pre-clinically evaluating novel therapies, prior to embarking upon lengthy and expensive clinical trials. Here, we describe the development of such a large animal model. A thoracic spinal cord injury at T10/11 was induced in Yucatan miniature pigs (20-25 kg) using a weight drop device. Varying degrees of injury severity were induced by altering the height of the weight drop (5, 10, 20, 30, 40, and 50 cm). Behavioral recovery over 12 weeks was measured using a newly developed Porcine Thoracic Injury Behavior Scale (PTIBS). This scale distinguished locomotor recovery among animals of different injury severities, with strong intra-observer and inter-observer reliability. Histological analysis of the spinal cords 12 weeks post-injury revealed that animals with the more biomechanically severe injuries had less spared white matter and gray matter and less neurofilament immunoreactivity. Additionally, the PTIBS scores correlated strongly with the extent of tissue sparing through the epicenter of injury. This large animal model of SCI may represent a useful intermediary in the testing of novel pharmacological treatments and cell transplantation strategies.
Keywords: Contusion; porcine; PTIBS; SCI
Rights: © Mary Ann Liebert, Inc.
RMID: 0030008117
DOI: 10.1089/neu.2012.2386
Appears in Collections:Medicine publications

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