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Type: Journal article
Title: Development of a surface to increase retinal pigment epithelial cell (ARPE-19) proliferation under reduced serum conditions
Author: Zuber, A.
Robinson, D.
Short, R.
Steele, D.
Whittle, J.
Citation: Journal of Materials Science: Materials in Medicine, 2014; 25(5):1367-1373
Publisher: Springer Verlag (Germany)
Issue Date: 2014
ISSN: 0957-4530
Statement of
Agnieszka A. Zuber, David E. Robinson, Robert D. Short, David A. Steele, Jason D. Whittle
Abstract: Age related macular degeneration of the eye is brought about by damage to the retinal pigment epithelium (RPE) and is a major cause of adult blindness. One potential treatment method is transplantation of RPE cells grown in vitro. Maintaining RPE cell viability and physiological function in vitro is a challenge, and this must also be achieved using materials that can be subsequently used to deliver an intact cell sheet into the eye. In this paper, plasma polymerisation has been used to develop a chemically modified surface for maintaining RPE cells in vitro. Multiwell plates modified with a plasma copolymer of allylamine and octadiene maintained RPE cell growth at a level similar to that of TCPS. However, the addition of bound glycosaminoglycans (GAGs) to the plasma polymerised surface significantly enhanced RPE proliferation. Simply adding GAG to the culture media had no positive effect. It is shown that a combination of plasma polymer and GAG is a promising method for developing suitable surfaces for cell growth and delivery, that can be applied to any substrate material.
Keywords: Cell Line
Epithelial Cells
Rights: © Springer Science+Business Media New York 2014
DOI: 10.1007/s10856-014-5163-y
Appears in Collections:Aurora harvest 7
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