Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/94664
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dc.contributor.authorCorbin, K.en
dc.contributor.authorByrt, C.en
dc.contributor.authorBauer, S.en
dc.contributor.authorDeBolt, S.en
dc.contributor.authorChambers, D.en
dc.contributor.authorHoltum, J.en
dc.contributor.authorKarem, G.en
dc.contributor.authorHenderson, M.en
dc.contributor.authorLahnstein, J.en
dc.contributor.authorBeahan, C.en
dc.contributor.authorBacic, A.en
dc.contributor.authorFincher, G.en
dc.contributor.authorBetts, N.en
dc.contributor.authorBurton, R.en
dc.date.issued2015en
dc.identifier.citationPLoS One, 2015; 10(8):e0135382-1-e0135382-23en
dc.identifier.issn1932-6203en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://hdl.handle.net/2440/94664-
dc.description.abstractPlant biomass from different species is heterogeneous, and this diversity in composition can be mined to identify materials of value to fuel and chemical industries. Agave produces high yields of energy-rich biomass, and the sugar-rich stem tissue has traditionally been used to make alcoholic beverages. Here, the compositions of Agave americana and Agave tequilana leaves are determined, particularly in the context of bioethanol production. Agave leaf cell wall polysaccharide content was characterized by linkage analysis, non-cellulosic polysaccharides such as pectins were observed by immuno-microscopy, and leaf juice composition was determined by liquid chromatography. Agave leaves are fruit-like-rich in moisture, soluble sugars and pectin. The dry leaf fiber was composed of crystalline cellulose (47-50% w/w) and non-cellulosic polysaccharides (16-22% w/w), and whole leaves were low in lignin (9-13% w/w). Of the dry mass of whole Agave leaves, 85-95% consisted of soluble sugars, cellulose, non-cellulosic polysaccharides, lignin, acetate, protein and minerals. Juice pressed from the Agave leaves accounted for 69% of the fresh weight and was rich in glucose and fructose. Hydrolysis of the fructan oligosaccharides doubled the amount of fermentable fructose in A. tequilana leaf juice samples and the concentration of fermentable hexose sugars was 41-48 g/L. In agricultural production systems such as the tequila making, Agave leaves are discarded as waste. Theoretically, up to 4000 L/ha/yr of bioethanol could be produced from juice extracted from waste Agave leaves. Using standard Saccharomyces cerevisiae strains to ferment Agave juice, we observed ethanol yields that were 66% of the theoretical yields. These data indicate that Agave could rival currently used bioethanol feedstocks, particularly if the fermentation organisms and conditions were adapted to suit Agave leaf composition.en
dc.description.statementofresponsibilityKendall R. Corbin, Caitlin S. Byrt, Stefan Bauer, Seth DeBolt, Don Chambers, Joseph A. M. Holtum, Ghazwan Karem, Marilyn Henderson, Jelle Lahnstein, Cherie T. Beahan, Antony Bacic, Geoffrey B. Fincher, Natalie S. Betts, Rachel A. Burtonen
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2015 Corbin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.subjectAgave; Plant Leaves; Cellulose; Lignin; Polysaccharides; Biomass; Fermentation; Hydrolysis; Renewable Energyen
dc.titleProspecting for energy-rich renewable raw materials: agave leaf case studyen
dc.typeJournal articleen
dc.identifier.rmid0030033892en
dc.identifier.doi10.1371/journal.pone.0135382en
dc.relation.granthttp://purl.org/au-research/grants/arc/CE1101007en
dc.identifier.pubid207347-
pubs.library.collectionAgriculture, Food and Wine publicationsen
pubs.library.teamDS01en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
Appears in Collections:Agriculture, Food and Wine publications

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