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Type: Journal article
Title: Residual soil DNA extraction increases the discriminatory power between samples
Author: Young, J.
Weyrich, L.
Clarke, L.
Cooper, A.
Citation: Forensic Science, Medicine, and Pathology, 2015; 11(2):268-272
Publisher: Springer
Issue Date: 2015
ISSN: 1547-769X
Statement of
Jennifer M. Young, Laura S. Weyrich, Laurence J. Clarke, Alan Cooper
Abstract: Forensic soil analysis relies on capturing an accurate and reproducible representation of the diversity from limited quantities of soil; however, inefficient DNA extraction can markedly alter the taxonomic abundance. The performance of a standard commercial DNA extraction kit (MOBIO PowerSoil DNA Isolation kit) and three modified protocols of this kit: soil pellet re-extraction (RE); an additional 24-h lysis incubation step at room temperature (RT); and 24-h lysis incubation step at 55°C (55) were compared using high-throughput sequencing of the internal transcribed spacer I ribosomal DNA. DNA yield was not correlated with fungal diversity and the four DNA extraction methods displayed distinct fungal community profiles for individual samples, with some phyla detected exclusively using the modified methods. Application of a 24 h lysis step will provide a more complete inventory of fungal biodiversity, and re-extraction of the residual soil pellet offers a novel tool for increasing discriminatory power between forensic soil samples.
Keywords: Soil; forensics; metagenomics; DNA extraction; high-throughput sequencing
Rights: © Springer Science+Business Media New York 2015
RMID: 0030024290
DOI: 10.1007/s12024-015-9662-z
Appears in Collections:Earth and Environmental Sciences publications

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