Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/94717
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Type: Journal article
Title: Distribution and Inhibition of liposomes on Staphylococcus aureus and Pseudomonas aeruginosa biofilm
Author: Dong, D.
Thomas, N.
Thierry, B.
Vreugde, S.
Prestidge, C.
Wormald, P.
Citation: PLoS One, 2015; 10(6):e0131806
Publisher: Public Library of Science
Issue Date: 2015
ISSN: 1932-6203
1932-6203
Statement of
Responsibility: 
Dong Dong, Nicky Thomas, Benjamin Thierry, Sarah Vreugde, Clive A. Prestidge, Peter-John Wormald
Abstract: BACKGROUND Staphylococcus aureus and Pseudomonas aeruginosa are major pathogens in chronic rhinosinusitis (CRS) and their biofilms have been associated with poorer postsurgical outcomes. This study investigated the distribution and anti-biofilm effect of cationic (+) and anionic (-) phospholipid liposomes with different sizes (unilamellar and multilamellar vesicle, ULV and MLV respectively) on S. aureus and P. aeruginosa biofilms. METHOD Specific biofilm models for S. aureus ATCC 25923 and P. aeruginosa ATCC 15692 were established. Liposomal distribution was determined by observing SYTO9 stained biofilm exposed to DiI labeled liposomes using confocal scanning laser microscopy, followed by quantitative image analysis. The anti-biofilm efficacy study was carried out by using the alamarBlue assay to test the relative viability of biofilm treated with various liposomes for 24 hours and five minutes. RESULTS The smaller ULVs penetrated better than larger MLVs in both S. aureus and P. aeruginosa biofilm. Except that +ULV and –ULV displayed similar distribution in S. aureus biofilm, the cationic liposomes adhered better than their anionic counterparts. Biofilm growth was inhibited at 24-hour and five-minute exposure time, although the decrease of viability for P. aeruginosa biofilm after liposomal treatment did not reach statistical significance. CONCLUSION The distribution and anti-biofilm effects of cationic and anionic liposomes of different sizes differed in S. aureus and P. aeruginosa biofilms. Reducing the liposome size and formulating liposomes as positively charged enhanced the penetration and inhibition of S. aureus and P. aeruginosa biofilms.
Keywords: Humans; Biofilms; Pseudomonas aeruginosa; Staphylococcus aureus; Pseudomonas Infections; Staphylococcal Infections; Phospholipids; Liposomes; Anti-Bacterial Agents; Microscopy, Confocal
Rights: © 2015 Dong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
RMID: 0030034772
DOI: 10.1371/journal.pone.0131806
Grant ID: http://purl.org/au-research/grants/nhmrc/1047576
Appears in Collections:Medicine publications

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