Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/94791
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Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Weyrich, L. | - |
dc.contributor.author | Rolin, O. | - |
dc.contributor.author | Muse, S. | - |
dc.contributor.author | Park, J. | - |
dc.contributor.author | Spidale, N. | - |
dc.contributor.author | Kennett, M. | - |
dc.contributor.author | Hester, S. | - |
dc.contributor.author | Chen, C. | - |
dc.contributor.author | Dudley, E. | - |
dc.contributor.author | Harvill, E. | - |
dc.contributor.editor | Boneca, I. | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | PLoS One, 2012; 7(10):e45892-1-e45892-12 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2440/94791 | - |
dc.description.abstract | Type VI Secretion Systems (T6SSs) have been identified in numerous gram-negative pathogens, but the lack of a natural host infection model has limited analysis of T6SS contributions to infection and pathogenesis. Here, we describe disruption of a gene within locus encoding a putative T6SS in Bordetella bronchiseptica strain RB50, a respiratory pathogen that circulates in a broad range of mammals, including humans, domestic animals, and mice. The 26 gene locus encoding the B. bronchiseptica T6SS contains apparent orthologs to all known core genes and possesses thirteen novel genes. By generating an in frame deletion of clpV, which encodes a putative ATPase required for some T6SS-dependent protein secretion, we observe that ClpV contributes to in vitro macrophage cytotoxicity while inducing several eukaryotic proteins associated with apoptosis. Additionally, ClpV is required for induction of IL-1β, IL-6, IL-17, and IL-10 production in J774 macrophages infected with RB50. During infections in wild type mice, we determined that ClpV contributes to altered cytokine production, increased pathology, delayed lower respiratory tract clearance, and long term nasal cavity persistence. Together, these results reveal a natural host infection system in which to interrogate T6SS contributions to immunomodulation and pathogenesis. | - |
dc.description.statementofresponsibility | Laura S. Weyrich, Olivier Y. Rolin, Sarah J. Muse, Jihye Park, Nicholas Spidale, Mary J. Kennett, Sara E. Hester, Chun Chen, Edward G. Dudley, Eric T. Harvill | - |
dc.language.iso | en | - |
dc.publisher | Public Library of Science | - |
dc.rights | © Weyrich et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | - |
dc.source.uri | http://dx.doi.org/10.1371/journal.pone.0045892 | - |
dc.subject | Macrophages | - |
dc.subject | Animals | - |
dc.subject | Mice | - |
dc.subject | Bordetella bronchiseptica | - |
dc.subject | Bordetella Infections | - |
dc.subject | Bacterial Proteins | - |
dc.subject | Interleukin-6 | - |
dc.subject | Interleukin-17 | - |
dc.subject | Virulence Factors | - |
dc.subject | Virulence | - |
dc.subject | Up-Regulation | - |
dc.subject | Multigene Family | - |
dc.subject | Adenosine Triphosphatases | - |
dc.subject | Interleukin-1beta | - |
dc.subject | Immunomodulation | - |
dc.title | A type VI secretion system encoding locus is required for bordetella bronchiseptica immunomodulation and persistence in vivo | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1371/journal.pone.0045892 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 7 Genetics publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_94791.pdf | Published version | 1.3 MB | Adobe PDF | View/Open |
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