Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/94876
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dc.contributor.authorNewman, M.en
dc.contributor.authorSykes, P.en
dc.contributor.authorBlyth, B.en
dc.contributor.authorBezak, E.en
dc.contributor.authorLawrence, M.en
dc.contributor.authorMorel, K.en
dc.contributor.authorOrmsby, R.en
dc.date.issued2014en
dc.identifier.citationPLoS One, 2014; 9(3):e93016-1-e93016-10en
dc.identifier.issn1932-6203en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://hdl.handle.net/2440/94876-
dc.description.abstractThe low dose radioadaptive response has been shown to be protective against high doses of radiation as well as aging-induced genomic instability. We hypothesised that a single whole-body exposure of low dose radiation would induce a radioadaptive response thereby reducing or abrogating aging-related changes in repeat element DNA methylation in mice. Following sham or 10 mGy X-irradiation, serial peripheral blood sampling was performed and differences in Long Interspersed Nucleic Element 1 (L1), B1 and Intracisternal-A-Particle (IAP) repeat element methylation between samples were assessed using high resolution melt analysis of PCR amplicons. By 420 days post-irradiation, neither radiation- or aging-related changes in the methylation of peripheral blood, spleen or liver L1, B1 and IAP elements were observed. Analysis of the spleen and liver tissues of cohorts of untreated aging mice showed that the 17-19 month age group exhibited higher repeat element methylation than younger or older mice, with no overall decline in methylation detected with age. This is the first temporal analysis of the effect of low dose radiation on repeat element methylation in mouse peripheral blood and the first to examine the long term effect of this dose on repeat element methylation in a radiosensitive tissue (spleen) and a tissue fundamental to the aging process (liver). Our data indicate that the methylation of murine DNA repeat elements can fluctuate with age, but unlike human studies, do not demonstrate an overall aging-related decline. Furthermore, our results indicate that a low dose of ionising radiation does not induce detectable changes to murine repeat element DNA methylation in the tissues and at the time-points examined in this study. This radiation dose is relevant to human diagnostic radiation exposures and suggests that a dose of 10 mGy X-rays, unlike high dose radiation, does not cause significant short or long term changes to repeat element or global DNA methylation.en
dc.description.statementofresponsibilityMichelle R. Newman, Pamela J. Sykes, Benjamin J. Blyth, Eva Bezak, Mark D. Lawrence, Katherine L. Morel, Rebecca J. Ormsbyen
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2014 Newman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectLiver; Spleen; Animals; Mice; Whole-Body Irradiation; Models, Animal; Radiation Dosage; Age Factors; DNA Methylation; Repetitive Sequences, Nucleic Acid; Genes, Intracisternal A-Particle; Long Interspersed Nucleotide Elements; X-Rays; Female; Maleen
dc.titleA single whole-body low dose X-irradiation does not affect L1, B1 and IAP repeat element DNA methylation longitudinallyen
dc.typeJournal articleen
dc.identifier.doi10.1371/journal.pone.0093016en
pubs.publication-statusPublisheden
dc.identifier.orcidBezak, E. [0000-0002-1315-1735]en
Appears in Collections:Physics publications

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