Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: The complete genome and phenome of a community-acquired acinetobacter baumannii
Author: Farrugia, D.
Elbourne, L.
Hassan, K.
Eijkelkamp, B.
Tetu, S.
Brown, M.
Shah, B.
Peleg, A.
Mabbutt, B.
Paulsen, I.
Citation: PLoS One, 2013; 8(3):e58628-1-e58628-13
Publisher: Public Library of Science
Issue Date: 2013
ISSN: 1932-6203
Statement of
Daniel N. Farrugia, Liam D. H. Elbourne, Karl A. Hassan, Bart A. Eijkelkamp, Sasha G. Tetu, Melissa H. Brown, Bhumika S. Shah, Anton Y. Peleg, Bridget C. Mabbutt, Ian T. Paulsen
Abstract: Many sequenced strains of Acinetobacter baumannii are established nosocomial pathogens capable of resistance to multiple antimicrobials. Community-acquired A. baumannii in contrast, comprise a minor proportion of all A. baumannii infections and are highly susceptible to antimicrobial treatment. However, these infections also present acute clinical manifestations associated with high reported rates of mortality. We report the complete 3.70 Mbp genome of A. baumannii D1279779, previously isolated from the bacteraemic infection of an Indigenous Australian; this strain represents the first community-acquired A. baumannii to be sequenced. Comparative analysis of currently published A. baumannii genomes identified twenty-four accessory gene clusters present in D1279779. These accessory elements were predicted to encode a range of functions including polysaccharide biosynthesis, type I DNA restriction-modification, and the metabolism of novel carbonaceous and nitrogenous compounds. Conversely, twenty genomic regions present in previously sequenced A. baumannii strains were absent in D1279779, including gene clusters involved in the catabolism of 4-hydroxybenzoate and glucarate, and the A. baumannii antibiotic resistance island, known to bestow resistance to multiple antimicrobials in nosocomial strains. Phenomic analysis utilising the Biolog Phenotype Microarray system indicated that A. baumannii D1279779 can utilise a broader range of carbon and nitrogen sources than international clone I and clone II nosocomial isolates. However, D1279779 was more sensitive to antimicrobial compounds, particularly beta-lactams, tetracyclines and sulphonamides. The combined genomic and phenomic analyses have provided insight into the features distinguishing A. baumannii isolated from community-acquired and nosocomial infections.
Keywords: Pyloric Antrum; Humans; Acinetobacter baumannii; Acinetobacter Infections; Community-Acquired Infections; DNA, Bacterial; DNA Transposable Elements; Oligonucleotide Array Sequence Analysis; Chromosome Mapping; Drug Resistance, Bacterial; Genome, Bacterial; Multigene Family
Rights: © 2013 Farrugia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0030012718
DOI: 10.1371/journal.pone.0058628
Grant ID:
Appears in Collections:Microbiology and Immunology publications

Files in This Item:
File Description SizeFormat 
hdl_94976.pdfPublished version1.45 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.