Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/94987
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dc.contributor.authorGray, L.en
dc.contributor.authorTurville, S.en
dc.contributor.authorHItchen, T.en
dc.contributor.authorCheng, W.en
dc.contributor.authorEllett, A.en
dc.contributor.authorSalimi, H.en
dc.contributor.authorRoche, M.en
dc.contributor.authorWesselingh, S.en
dc.contributor.authorGorry, P.en
dc.contributor.authorChurchill, M.en
dc.date.issued2014en
dc.identifier.citationPLoS One, 2014; 9(2):e90620-1-e90620-8en
dc.identifier.issn1932-6203en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://hdl.handle.net/2440/94987-
dc.description.abstractAstrocytes are extensively infected with HIV-1 in vivo and play a significant role in the development of HIV-1-associated neurocognitive disorders. Despite their extensive infection, little is known about how astrocytes become infected, since they lack cell surface CD4 expression. In the present study, we investigated the fate of HIV-1 upon infection of astrocytes. Astrocytes were found to bind and harbor virus followed by biphasic decay, with HIV-1 detectable out to 72 hours. HIV-1 was observed to associate with CD81-lined vesicle structures. shRNA silencing of CD81 resulted in less cell-associated virus but no loss of co-localization between HIV-1 and CD81. Astrocytes supported trans-infection of HIV-1 to T-cells without de novo virus production, and the virus-containing compartment required 37°C to form, and was trypsin-resistant. The CD81 compartment observed herein, has been shown in other cell types to be a relatively protective compartment. Within astrocytes, this compartment may be actively involved in virus entry and/or spread. The ability of astrocytes to transfer virus, without de novo viral synthesis suggests they are capable of sequestering and protecting virus and thus, they could potentially facilitate viral dissemination in the CNS.en
dc.description.statementofresponsibilityLachlan R. Gray, Stuart G. Turville, Tina L. HItchen, Wan-Jung Cheng, AnneM. Ellett, Hamid Salimi, Michael J. Roche, Steve L. Wesselingh, Paul R. Gorry, Melissa J. Churchillen
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2014 Gray et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectAstrocytes; T-Lymphocytes; Cell Line; Transport Vesicles; Humans; HIV-1; Microscopy, Fluorescence; Coculture Techniques; Temperature; Virus Replication; RNA Interference; Protein Binding; Time Factors; Virus Internalization; Host-Pathogen Interactions; HEK293 Cells; Antigens, CD81en
dc.titleHIV-1 entry and trans-infection of astrocytes involves CD81 vesiclesen
dc.typeJournal articleen
dc.identifier.rmid0030026860en
dc.identifier.doi10.1371/journal.pone.0090620en
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1051093en
dc.identifier.pubid114646-
pubs.library.collectionMedicine publicationsen
pubs.library.teamDS01en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
Appears in Collections:Medicine publications

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