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Type: Journal article
Title: Altered formalin-induced pain and fos induction in the periaqueductal grey of preadolescent rats following neonatal LPS exposure
Author: Zouikr, I.
James, M.
Campbell, E.
Clifton, V.
Beagley, K.
Dayas, C.
Hodgson, D.
Citation: PLoS One, 2014; 9(5):e98382-1-e98382-8
Publisher: Public Library of Science
Issue Date: 2014
ISSN: 1932-6203
Editor: Siegel, A.
Statement of
Ihssane Zouikr, Morgan H. James, Erin J. Campbell, Vicki L. Clifton, Kenneth W. Beagley, Christopher V. Dayas, Deborah M. Hodgson
Abstract: Animal and human studies have demonstrated that early pain experiences can produce alterations in the nociceptive systems later in life including increased sensitivity to mechanical, thermal, and chemical stimuli. However, less is known about the impact of neonatal immune challenge on future responses to noxious stimuli and the reactivity of neural substrates involved in analgesia. Here we demonstrate that rats exposed to Lipopolysaccharide (LPS; 0.05 mg/kg IP, Salmonella enteritidis) during postnatal day (PND) 3 and 5 displayed enhanced formalin-induced flinching but not licking following formalin injection at PND 22. This LPS-induced hyperalgesia was accompanied by distinct recruitment of supra-spinal regions involved in analgesia as indicated by significantly attenuated Fos-protein induction in the rostral dorsal periaqueductal grey (DPAG) as well as rostral and caudal axes of the ventrolateral PAG (VLPAG). Formalin injections were associated with increased Fos-protein labelling in lateral habenula (LHb) as compared to medial habenula (MHb), however the intensity of this labelling did not differ as a result of neonatal immune challenge. These data highlight the importance of neonatal immune priming in programming inflammatory pain sensitivity later in development and highlight the PAG as a possible mediator of this process.
Keywords: Periaqueductal Gray
Spinal Cord
Animals, Newborn
Rats, Wistar
Proto-Oncogene Proteins c-fos
Pain Threshold
Rights: © 2014 Zouikr et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: 10.1371/journal.pone.0098382
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