Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/95019
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Type: Journal article
Title: The X-Linked Inhibitor of Apoptosis Protein inhibitor embelin suppresses inflammation and bone erosion in collagen antibody induced arthritis mice
Author: Dharmapatni, A.
Cantley, M.
Marino, V.
Perilli, E.
Crotti, T.
Smith, M.
Haynes, D.
Citation: Mediators of Inflammation, 2015; 2015:564042-1-564042-10
Publisher: Hindawi Publishing Corporation
Issue Date: 2015
ISSN: 0962-9351
1466-1861
Statement of
Responsibility: 
Anak A. S. S. K. Dharmapatni, Melissa D. Cantley, Victor Marino, Egon Perilli, Tania N. Crotti, Malcolm D. Smith, and David R. Haynes
Abstract: Objective. To investigate the effect of Embelin, an inhibitor of X-Linked Inhibitor of Apoptosis Protein (XIAP), on inflammation and bone erosion in a collagen antibody induced arthritis (CAIA) in mice. Methods. Four groups of mice (n = 6 per group) were allocated: CAIA untreated mice, CAIA treated with Prednisolone (10 mg/kg/day), CAIA treated with low dose Embelin (30 mg/kg/day), and CAIA treated with high dose Embelin (50 mg/kg/day). Joint inflammation was evaluated using clinical paw score and histological assessments. Bone erosion was assessed using micro-CT, tartrate resistant acid phosphatase (TRAP) staining, and serum carboxy-terminal collagen crosslinks (CTX-1) ELISA. Immunohistochemistry was used to detect XIAP protein. TUNEL was performed to identify apoptotic cells. Results. Low dose, but not high dose Embelin, suppressed inflammation as reflected by lower paw scores (P < 0.05) and lower histological scores for inflammation. Low dose Embelin reduced serum CTX-1 (P < 0.05) and demonstrated lower histological score and TRAP counting, and slightly higher bone volume as compared to CAIA untreated mice. XIAP expression was not reduced but TUNEL positive cells were more abundant in Embelin treated CAIA mice. Conclusion. Low dose Embelin suppressed inflammation and serum CTX-1 in CAIA mice, indicating a potential use for Embelin to treat pathological bone loss.
Keywords: Animals; Mice; Bone Resorption; Arthritis, Experimental; Inflammation; Benzoquinones; Acid Phosphatase; Isoenzymes; X-Linked Inhibitor of Apoptosis Protein; Tartrate-Resistant Acid Phosphatase
Description: Research article
Rights: © 2015 Anak A. S. S. K. Dharmapatni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0030023875
DOI: 10.1155/2015/564042
Appears in Collections:Medicine publications

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