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https://hdl.handle.net/2440/95049
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Type: | Journal article |
Title: | Analysis of high pI α-Amy-1 gene family members expressed in late maturity α-amylase in wheat (Triticum aestivum L.) |
Other Titles: | Analysis of high pI alpha-Amy-1 gene family members expressed in late maturity alpha-amylase in wheat (Triticum aestivum L.) |
Author: | Cheng, C. Oldach, K. Mrva, K. Mares, D. |
Citation: | Molecular Breeding: new strategies in plant improvement, 2014; 33(3):519-529 |
Publisher: | Springer |
Issue Date: | 2014 |
ISSN: | 1380-3743 1572-9788 |
Statement of Responsibility: | Cong-Rong Cheng, Klaus Oldach, Kolumbina Mrva, Daryl Mares |
Abstract: | Late maturity α-amylase (LMA) is a genetic defect involving the synthesis of high pI isozymes of α-amylase encoded by α-Amy-1 genes during the later stages of grain development. The aims of this investigation were to determine both the number of expressed α-Amy-1 genes and their relative transcript abundance. Sub-cloning and sequencing of expressed high pI α-amylase genes in developing wheat seeds revealed three insertion/deletion patterns in the 3' untranslated region and numerous single nucleotide polymorphisms at the 3' end of α-Amy-1. The genetic variations defined 36 α-Amy-1 gene sequences that were expressed on the onset of LMA in doubled haploid progenies (SpM25, SpM52 and SpM127) derived from the cross Spica (LMA)/Maringa (non-LMA). Five isoelectric point groups were predicted based on the translated partial coding sequences. The potential application of quantitative real-time RT-PCR in screening wheat genotypes for LMA is discussed. |
Keywords: | High pI α-amylase Late maturity α-amylase (LMA) Quantitative real-time RT-PCR Triticum aestivum Wheat α-Amy-1 genes |
Rights: | © The Author(s) 2013. This article is published with open access at Springerlink.com |
DOI: | 10.1007/s11032-013-9968-z |
Published version: | http://dx.doi.org/10.1007/s11032-013-9968-z |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 3 |
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