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|Title:||Analysis of high pI α-Amy-1 gene family members expressed in late maturity α-amylase in wheat (Triticum aestivum L.)|
|Other Titles:||Analysis of high pI alpha-Amy-1 gene family members expressed in late maturity alpha-amylase in wheat (Triticum aestivum L.)|
|Citation:||Molecular Breeding, 2014; 33(3):519-529|
|Cong-Rong Cheng, Klaus Oldach, Kolumbina Mrva, Daryl Mares|
|Abstract:||Late maturity α-amylase (LMA) is a genetic defect involving the synthesis of high pI isozymes of α-amylase encoded by α-Amy-1 genes during the later stages of grain development. The aims of this investigation were to determine both the number of expressed α-Amy-1 genes and their relative transcript abundance. Sub-cloning and sequencing of expressed high pI α-amylase genes in developing wheat seeds revealed three insertion/deletion patterns in the 3' untranslated region and numerous single nucleotide polymorphisms at the 3' end of α-Amy-1. The genetic variations defined 36 α-Amy-1 gene sequences that were expressed on the onset of LMA in doubled haploid progenies (SpM25, SpM52 and SpM127) derived from the cross Spica (LMA)/Maringa (non-LMA). Five isoelectric point groups were predicted based on the translated partial coding sequences. The potential application of quantitative real-time RT-PCR in screening wheat genotypes for LMA is discussed.|
|Keywords:||High pI α-amylase; Late maturity α-amylase (LMA); Quantitative real-time RT-PCR; Triticum aestivum; Wheat; α-Amy-1 genes|
|Rights:||© The Author(s) 2013. This article is published with open access at Springerlink.com|
|Appears in Collections:||Agriculture, Food and Wine publications|
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