Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/95049
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Type: Journal article
Title: Analysis of high pI α-Amy-1 gene family members expressed in late maturity α-amylase in wheat (Triticum aestivum L.)
Other Titles: Analysis of high pI alpha-Amy-1 gene family members expressed in late maturity alpha-amylase in wheat (Triticum aestivum L.)
Author: Cheng, C.
Oldach, K.
Mrva, K.
Mares, D.
Citation: Molecular Breeding, 2014; 33(3):519-529
Publisher: Springer
Issue Date: 2014
ISSN: 1380-3743
1572-9788
Statement of
Responsibility: 
Cong-Rong Cheng, Klaus Oldach, Kolumbina Mrva, Daryl Mares
Abstract: Late maturity α-amylase (LMA) is a genetic defect involving the synthesis of high pI isozymes of α-amylase encoded by α-Amy-1 genes during the later stages of grain development. The aims of this investigation were to determine both the number of expressed α-Amy-1 genes and their relative transcript abundance. Sub-cloning and sequencing of expressed high pI α-amylase genes in developing wheat seeds revealed three insertion/deletion patterns in the 3' untranslated region and numerous single nucleotide polymorphisms at the 3' end of α-Amy-1. The genetic variations defined 36 α-Amy-1 gene sequences that were expressed on the onset of LMA in doubled haploid progenies (SpM25, SpM52 and SpM127) derived from the cross Spica (LMA)/Maringa (non-LMA). Five isoelectric point groups were predicted based on the translated partial coding sequences. The potential application of quantitative real-time RT-PCR in screening wheat genotypes for LMA is discussed.
Keywords: High pI α-amylase; Late maturity α-amylase (LMA); Quantitative real-time RT-PCR; Triticum aestivum; Wheat; α-Amy-1 genes
Rights: © The Author(s) 2013. This article is published with open access at Springerlink.com
RMID: 0030010256
DOI: 10.1007/s11032-013-9968-z
Appears in Collections:Agriculture, Food and Wine publications

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