Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Resident microbiota affect bordetella pertussis infectious dose and host specificity
Author: Weyrich, L.
Feaga, H.
Park, J.
Muse, S.
Safi, C.
Rolin, O.
Young, S.
Harvill, E.
Citation: Journal of Infectious Diseases, 2014; 209(6):913-921
Publisher: Oxford University Press
Issue Date: 2014
ISSN: 0022-1899
Statement of
Laura S. Weyrich, Heather A. Feaga, Jihye Park, Sarah J. Muse, Chetan Y. Safi, Olivier Y. Rolin, Sarah E. Young and Eric T. Harvill
Abstract: Before contacting host tissues, invading pathogens directly or indirectly interact with host microbiota, but the effects of such interactions on the initial stages of infection are poorly understood. Bordetella pertussis is highly infectious among humans but requires large doses to colonize rodents, unlike a closely related zoonotic pathogen, Bordetella bronchiseptica, raising important questions about the contributions of bacterial competition to initial colonization and host selection. We observed that <100 colony-forming units (CFU) of B. bronchiseptica efficiently infected mice and displaced culturable host microbiota, whereas 10 000 CFU of B. pertussis were required to colonize murine nasal cavities and did not displace host microorganisms. Bacteria isolated from murine nasal cavities but not those from the human lower respiratory tract limited B. pertussis growth in vitro, indicating that interspecies competition may limit B. pertussis colonization of mice. Further, a broad-spectrum antibiotic treatment delivered before B. pertussis inoculation reduced the infectious dose to <100 CFU, and reintroduction of single Staphylococcus or Klebsiella species was sufficient to inhibit B. pertussis colonization of antibiotic-treated mice. Together, these results reveal that resident microorganisms can prevent B. pertussis colonization and influence host specificity, and they provide rationale for manipulating microbiomes to create more-accurate animal models of infectious diseases.
Keywords: microbiome; Bordetella; antibiotics; whooping cough; pathogen evolution; host adaptation; interspecies competition; bacterial competition
Rights: © The Author 2013
RMID: 0030019154
DOI: 10.1093/infdis/jit597
Appears in Collections:Molecular and Biomedical Science publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.