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Type: Journal article
Title: Constitutive overexpression of soybean plasma membrane intrinsic protein GmPIP1;6 confers salt tolerance
Author: Zhou, L.
Wang, C.
Liu, R.
Han, Q.
Vandeleur, R.
Du, J.
Tyerman, S.
Shou, H.
Citation: BMC Plant Biology, 2014; 14(1):181-1-181-13
Publisher: BioMed Central Ltd
Issue Date: 2014
ISSN: 1471-2229
Statement of
Lian Zhou, Chuang Wang, Ruifang Liu, Qiang Han, Rebecca K Vandeleur, Juan Du, Steven Tyerman and Huixia Shou
Abstract: BACKGROUND: Under saline conditions, plant growth is depressed via osmotic stress and salt can accumulate in leaves leading to further depression of growth due to reduced photosynthesis and gas exchange. Aquaporins are proposed to have a major role in growth of plants via their impact on root water uptake and leaf gas exchange. In this study, soybean plasma membrane intrinsic protein 1;6 (GmPIP1;6) was constitutively overexpressed to evaluate the function of GmPIP1;6 in growth regulation and salt tolerance in soybean. RESULTS: GmPIP1;6 is highly expressed in roots as well as reproductive tissues and the protein targeted to the plasma membrane in onion epidermis. Treatment with 100 mM NaCl resulted in reduced expression initially, then after 3 days the expression was increased in root and leaves. The effects of constitutive overexpression of GmPIP1;6 in soybean was examined under normal and salt stress conditions. Overexpression in 2 independent lines resulted in enhanced leaf gas exchange, but not growth under normal conditions compared to wild type (WT). With 100 mM NaCl, net assimilation was much higher in the GmPIP1;6-Oe and growth was enhanced relative to WT. GmPIP1;6-Oe plants did not have higher root hydraulic conductance (Lo) under normal conditions, but were able to maintain Lo under saline conditions compared to WT which decreased Lo. GmPIP1;6-Oe lines grown in the field had increased yield resulting mainly from increased seed size. CONCLUSIONS: The general impact of overexpression of GmPIP1;6 suggests that it may be a multifunctional aquaporin involved in root water transport, photosynthesis and seed loading. GmPIP1;6 is a valuable gene for genetic engineering to improve soybean yield and salt tolerance.
Keywords: Soybean; aquaporins; salt tolerance; ovexpression; transformation; GmPIP1;6
Rights: © 2014 Zhou et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.
DOI: 10.1186/1471-2229-14-181
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