Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/95767
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Type: Journal article
Title: Expression of genes involved in the T cell signalling pathway in circulating immune cells of cattle 24 months following oral challenge with Bovine Amyloidotic Spongiform Encephalopathy (BASE)
Author: Trovato, A.
Panelli, S.
Strozzi, F.
Cambulli, C.
Barbieri, I.
Martinelli, N.
Lombardi, G.
Capoferri, R.
Williams, J.
Citation: BMC Veterinary Research, 2015; 11(1):105-1-105-9
Publisher: Biomed Central
Issue Date: 2015
ISSN: 1746-6148
1746-6148
Statement of
Responsibility: 
Andrea Trovato, Simona Panelli, Francesco Strozzi, Caterina Cambulli, Ilaria Barbieri, Nicola Martinelli, Guerino Lombardi, Rossana Capoferri and John L Williams
Abstract: BACKGROUND: Bovine Amyloidotic Spongiform Encephalopathy (BASE) is a variant of classical BSE that affects cows and can be transmitted to primates and mice. BASE is biochemically different from BSE and shares some molecular and histo-pathological features with the MV2 sub-type of human sporadic Creutzfeld Jakob Disease (sCJD). RESULTS: The present work examined the effects of BASE on gene expression in circulating immune cells. Ontology analysis of genes differentially expressed between cattle orally challenged with brain homogenate from cattle following intracranial inoculation with BASE and control cattle identified three main pathways which were affected. Within the immune function pathway, the most affected genes were related to the T cell receptor-mediated T cell activation pathways. The differential expression of these genes in BASE challenged animals at 10,12 and 24 months following challenge, vs unchallenged controls, was investigated by real time PCR. CONCLUSIONS: The results of this study show that the effects of prion diseases are not limited to the CNS, but involve the immune system and particularly T cell signalling during the early stage following challenge, before the appearance of clinical signs.
Keywords: Bovine Amyloidotic Spongiform Encephalopathy (BASE); Transmissible Spongiform Encephalopathies (TSEs); Immune function; Cattle
Rights: © 2015 Trovato et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
RMID: 0030031020
DOI: 10.1186/s12917-015-0412-y
Appears in Collections:Animal and Veterinary Sciences publications

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