Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/9577
Citations
Scopus Web of ScienceĀ® Altmetric
?
?
Type: Journal article
Title: Substrate proteolysis is inhibited by dominant-negative Nedd4 and Rsp5 mutants harboring alterations in WW domain 1
Author: Shcherbik, N.
Kumar, S.
Haines, D.
Citation: Journal of Cell Science, 2002; 115(5):1041-1048
Publisher: Company of Biologists Ltd
Issue Date: 2002
ISSN: 0021-9533
1477-9137
Statement of
Responsibility: 
Natalia Shcherbik, Sharad Kumar and Dale S. Haines
Abstract: Mammalian Nedd4 and its budding yeast orthologue Rsp5 are members of a large family of HECT-domain-containing ubiquitin ligases. Besides possessing a Ca2+/lipid-binding domain, both ligases have multiple protein-interacting modules termed WW domains. The C-terminal WW domains mediate interactions with substrates, but the function of the first WW domain remains unclear. We found that expression of a WW domain 1 Nedd4 mutant inhibits the growth of budding yeast by affecting the rsp5-ole1 pathway. The WW domain 1 mutant-induced phenotype is suppressed by ole1 cDNA overexpression or oleic acid supplementation of growth media and ole1 RNA levels are reduced in cells expressing this Nedd4 mutant. Also, the WW domain 1 Nedd4 mutant associates via WW domains 2 and 3 with Spt23, a Rsp5 target and ole1 transactivator. The dominant-negative activity of this mutant is associated with promoting accumulation of unprocessed Spt23 and inhibiting generation of processed and presumably active protein. Also, Spt23 processing is inhibited by a Nedd4 mutant that lacks ubiquitin ligase activity and Spt23-binding-competent Rsp5 mutants harboring WW domain 1 or ligase domain mutations. Interestingly, in mammalian cells, wild-type Nedd4 promotes proteasome-mediated degradation of the precursor form of Spt23. WW domain 1 and ligase domain Nedd4 mutants block its degradation. These results indicate that WW domain 1 of these ligases interacts with cofactors that are required for ubiquitin/proteasome-dependent proteolysis of bound substrates.
Keywords: Nedd4
Rsp5
WW domain
ole1
Spt23
DOI: 10.1242/jcs.115.5.1041
Published version: http://jcs.biologists.org/cgi/content/abstract/115/5/1041
Appears in Collections:Aurora harvest
Medicine publications

Files in This Item:
File Description SizeFormat 
hdl_9577.pdf272.56 kBPublisher's PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.