Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/95871
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Type: Journal article
Title: The interferon-induced transmembrane proteins, IFITM1, IFITM2 and IFITM3 inhibit hepatitis C virus entry
Author: Narayana, S.
Helbig, K.
McCartney, E.
Eyre, N.
Bull, R.
Eltahla, A.
Lloyd, A.
Beard, M.
Citation: Journal of Biological Chemistry, 2015; 290(43):25946-25959
Publisher: American Society for Biochemistry and Molecular Biology
Issue Date: 2015
ISSN: 0021-9258
1083-351X
Statement of
Responsibility: 
Sumudu K. Narayana, Karla J. Helbig, Erin M. McCartney, Nicholas S. Eyre, Rowena A. Bull, Auda Eltahla, Andrew R. Lloyd and Michael R. Beard
Abstract: The IFITM family of proteins have recently been identified as important host effector molecules of the type I interferon response against viruses. IFITM1 has been identified as a potent antiviral effector against HCV, while the related family members IFITM2 and IFITM3 have been described to have antiviral effects against a broad range of RNA viruses. Here, we demonstrate that IFITM2 and IFITM3 play an integral role in the interferon response against HCV and act at the level of late entry stages of HCV infection. We have established that in hepatocytes, IFITM2 and IFITM3 localise to the late and early endosomes respectively, as well as the lysosome. Furthermore, we have demonstrated that S-palmitoylation of all three IFITM proteins is essential for anti-HCV activity, whilst the conserved tyrosine residue in the NTD of IFITM2 and IFITM3 plays a significant role in protein localisation. However, this tyrosine was found to be dispensable for anti-HCV activity, with mutation of the tyrosine resulting in an IFITM1-like phenotype with the retention of anti-HCV activity and co-localisation of IFITM2 and IFITM3 with CD81. In conclusion, we propose that the IFITM proteins act in a coordinated manner to restrict HCV infection by targeting the endocytosed HCV virion for lysosomal degradation and demonstrate that the actions of the IFITM proteins are indeed virus and cell-type specific.
Keywords: Hepatitis C virus (HCV); Interferon-induced transmembrane (IFITM)proteins; cellular immune response; interferon; protein palmitoylation; protein phosphorylation
Rights: © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.
RMID: 0030037139
DOI: 10.1074/jbc.M115.657346
Grant ID: http://purl.org/au-research/grants/nhmrc/1053206
http://purl.org/au-research/grants/nhmrc/626906
Appears in Collections:Microbiology and Immunology publications

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