Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/95926
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Type: Journal article
Title: Dissection of the hierarchy and synergism of the bile derived signal on Cryptosporidium parvum excystation and infectivity
Author: King, B.
Keegan, A.
Phillips, R.
Fanok, S.
Monis, P.
Citation: Parasitology (Cambridge), 2012; 139(12):1533-1546
Publisher: Cambridge University Press
Issue Date: 2012
ISSN: 0031-1820
1469-8161
Statement of
Responsibility: 
B. J. King, A. R. Keegan, R. Phillips, S. Fanok and P. T. Monis
Abstract: Bile salts have been identified as an important trigger for excystation of Cryptosporidium oocysts but the hierarchy or synergism of this signal in relation to other triggers involved in excystation is poorly understood. In addition to excystation, bile salts have also been reported to increase the invasiveness of sporozoites within in vitro culture, possibly by affecting the secretory pathway via modification of intracellular calcium signalling. Nevertheless, incorporation of bile or bile salts into in vitro assays is not universal, with recent reports of negative effects on parasite growth. Here we report that bile and sodium taurocholate significantly affect both excystation rate and parasite in vitro growth. We demonstrate that their effect on excystation is dose, time and pre-treatment temperature dependent, while increases in parasite replication appear to be associated with modulation of parasite intracellular calcium and increased host cell susceptibility to infection. Notably, we illustrate that bile has a significant effect on host cells and can be cytotoxic at concentrations not much higher than those currently used for in vitro assays. This work should assist with more rational design of in vitro culture systems, with significant considerations for assay format when incorporating bile or bile salts as an excystation trigger.
Keywords: Cryptosporidium; bile acids; excystation; in vitro infectivity; intracellular calcium
Rights: © Cambridge University Press 2012
RMID: 0030025396
DOI: 10.1017/S0031182012000984
Appears in Collections:Molecular and Biomedical Science publications

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