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Type: Journal article
Title: Src homology 2 domain-containing protein-tyrosine phosphatases, SHP-1 and SHP-2, are required for platelet endothelial cell adhesion molecule-1/CD31-mediated inhibitory signaling
Author: Henshall, T.
Jones, K.
Wilkinson, R.
Jackson, D.
Citation: Journal of Immunology, 2001; 166(5):3098-3106
Publisher: Amer Assoc Immunologists
Issue Date: 2001
ISSN: 0022-1767
Abstract: Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) is a newly assigned member of the Ig immunoreceptor tyrosine-based inhibitory motif superfamily, and its functional role is suggested to be an inhibitory receptor that modulates immunoreceptor tyrosine-based activation motif-dependent signaling cascades. To test whether PECAM-1 is capable of delivering inhibitory signals in B cells and the functional requirement of protein-tyrosine phosphatases (PTPs) for this inhibitory signaling, we generated chimeric Fc gamma RIIB1-PECAM-1 receptors containing the extracellular and transmembrane portions of murine Fc gamma RIIB1 and the cytoplasmic domain of human PECAM-1. These chimeric receptors were stably expressed in chicken DT40 B cells either as wild-type or mutant cells deficient in SHP-1(-/-), SHP-2(-/-), SHIP(-/-), or SHP-1/2(-/-) and then assessed for their ability to inhibit B cell Ag receptor (BCR) signaling. Coligation of wild-type Fc gamma RIIB1-PECAM-1 with BCR resulted in inhibition of intracellular calcium release, suggesting that the cytoplasmic domain of PECAM-1 is capable of delivering an inhibitory signal that blocks BCR-mediated activation. This PECAM-1-mediated inhibitory signaling correlated with tyrosine phosphorylation of the Fc gamma RIIB1-PECAM-1 chimera, recruitment of SHP-1 and SHP-2 PTPs by the phosphorylated chimera, and attenuation of calcium mobilization responses. Mutational analysis of the two tyrosine residues, 663 and 686, constituting the immunoreceptor tyrosine-based inhibitory motifs in PECAM-1 revealed that both tyrosine residues play a crucial role in the inhibitory signal. Functional analysis of various PTP-deficient DT40 B cell lines stably expressing wild-type chimeric Fc gamma RIIB1-PECAM-1 receptor indicated that cytoplasmic Src homology 2-domain-containing phosphatases, SHP-1 and SHP-2, were both necessary and sufficient to deliver inhibitory negative regulation upon coligation of BCR complex with inhibitory receptor.
Keywords: Animals
Cell Line
Intracellular Signaling Peptides and Proteins
Antigens, CD31
Recombinant Fusion Proteins
Signal Transduction
Lymphocyte Activation
Consensus Sequence
Protein Structure, Tertiary
src Homology Domains
Amino Acid Motifs
Protein Tyrosine Phosphatases
Protein Tyrosine Phosphatase, Non-Receptor Type 6
Protein Tyrosine Phosphatase, Non-Receptor Type 11
SH2 Domain-Containing Protein Tyrosine Phosphatases
Receptors, IgG
DOI: 10.4049/jimmunol.166.5.3098
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Appears in Collections:Aurora harvest
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