Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/96275
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Type: Journal article
Title: Comparative analysis of surface-exposed virulence factors of Acinetobacter baumannii
Author: Eijkelkamp, B.
Stroeher, U.
Hassan, K.
Paulsen, I.
Brown, M.
Citation: BMC Genomics, 2014; 15(1):1020-1-1020-12
Publisher: BioMed Central
Issue Date: 2014
ISSN: 1471-2164
1471-2164
Statement of
Responsibility: 
Bart A Eijkelkamp, Uwe H Stroeher, Karl A Hassan, Ian T Paulsen, and Melissa H Brown
Abstract: BACKGROUND: Acinetobacter baumannii is a significant hospital pathogen, particularly due to the dissemination of highly multidrug resistant isolates. Genome data have revealed that A. baumannii is highly genetically diverse, which correlates with major variations seen at the phenotypic level. Thus far, comparative genomic studies have been aimed at identifying resistance determinants in A. baumannii. In this study, we extend and expand on these analyses to gain greater insight into the virulence factors across eight A. baumannii strains which are clonally, temporally and geographically distinct, and includes an isolate considered non-pathogenic and a community-acquired A. baumannii. RESULTS: We have identified a large number of genes in the A. baumannii genomes that are known to play a role in virulence in other pathogens, such as the recently studied proline-alanine-alanine-arginine (PAAR)-repeat domains of the type VI secretion systems. Not surprising, many virulence candidates appear to be part of the A. baumannii core genome of virulent isolates but were often found to be insertionally disrupted in the avirulent A. baumannii strain SDF. Our study also reveals that many known or putative virulence determinants are restricted to specific clonal lineages, which suggests that these virulence determinants may be crucial for the success of these widespread common clones. It has previously been suggested that the high level of intrinsic and adaptive resistance has enabled the widespread presence of A. baumannii in the hospital environment. This appears to have facilitated the expansion of its repertoire of virulence traits, as in general, the nosocomial strains in this study possess more virulence genes compared to the community-acquired isolate. CONCLUSIONS: Major genetic variation in known or putative virulence factors was seen across the eight strains included in this study, suggesting that virulence mechanisms are complex and multifaceted in A. baumannii. Overall, these analyses increase our understanding of A. baumannii pathogenicity and will assist in future studies determining the significance of virulence factors within clonal lineages and/or across the species.
Keywords: Genomics; Virulome; Type VI secretion systems; Membrane
Rights: © 2014 Eijkelkamp et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
RMID: 0030016475
DOI: 10.1186/1471-2164-15-1020
Grant ID: http://purl.org/au-research/grants/nhmrc/535053
Appears in Collections:Molecular and Biomedical Science publications

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