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Type: Journal article
Title: Meta-analysis of two genome-wide association studies of bovine paratuberculosis
Author: Minozzi, G.
Williams, J.
Stella, A.
Strozzi, F.
Luini, M.
Settles, M.
Taylor, J.
Whitlock, R.
Zanella, R.
Neibergs, H.
Citation: PLoS One, 2012; 7(3):e32578-1-e32578-8
Publisher: Public Library of Science
Issue Date: 2012
ISSN: 1932-6203
Statement of
Giulietta Minozzi, John L. Williams, Alessandra Stella, Francesco Strozzi, Mario Luini, Matthew L. Settles, Jeremy F. Taylor, Robert H. Whitlock, Ricardo Zanella, Holly L. Neibergs
Abstract: BACKGROUND: Bovine paratuberculosis (ParaTB) also known as Johne's disease, is a contagious fatal disease resulting from infection by Mycobacterium avium subspecies paratuberculosis (MAP). Previous studies have identified loci associated with ParaTB using different measurements to define cases and controls. The objective of this study was to combine the data from two recent studies to identify genetic loci associated with MAP tissue infection and humoral immune response, defined by MAP ELISA-positive cattle, by comparing cases and control animals for one or both measures of infection. METHODOLOGY/PRINCIPAL FINDINGS: The two populations used for the association analyses were a cohort of MAP tissue infected animals and control Holstein cows from the USA and the second cohort composed of ELISA-positive and ELISA-negative Holstein cows from Italy. Altogether 1190 cattle were genotyped with the Illumina BovineSNP50 BeadChip. SNP markers were removed if the minor allele frequency <0.01 or genotyping failure was >5%. Animals were removed with >5% genotyping failure. Whole genome association analyses were conducted with the GRAMMAR-CG method using two different definitions of control populations. CONCLUSION/SIGNIFICANCE: The analyses identified several loci (P<5 e-05) associated with ParaTB, defined by positive ELISA and presence of bacteria in tissue compared to ELISA and tissue negative animals, on chromosomes 1, 12 and 15 and one unassigned SNP. These results confirmed associations on chromosome 12 and the unassigned SNP with ParaTB which had been found in the Italian population alone. Furthermore, several additional genomic regions were found associated with ParaTB when ELISA and tissue positive animals were compared with tissue negative samples. These loci were on chromosomes 1, 6, 7, 13, 16, 21,23 and 25 (P<5 e-05). The results clearly indicate the importance of the phenotype definition when seeking to identify markers associated with different disease responses.
Keywords: Animals
Rights: © 2012 Minozzi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0030037595
DOI: 10.1371/journal.pone.0032578
Appears in Collections:Animal and Veterinary Sciences publications

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