Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/97131
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Type: Journal article
Title: Immune profiling in human breast cancer using high-sensitivity detection and analysis techniques
Author: Coventry, B.
Weightman, M.
Bradley, J.
Skinner, J.
Citation: JRSM Open, 2015; 6(9):1-12
Publisher: SAGE
Issue Date: 2015
ISSN: 2054-2704
2054-2704
Statement of
Responsibility: 
Brendon J Coventry, Michael J Weightman, John Bradley and John M Skinner
Abstract: OBJECTIVES: Evaluation of immune profiles in human breast cancer using high-sensitivity detection and analysis methods. DESIGN: Cohort comparative analysis studies of breast tissue. SETTING: Human hospital and laboratory healthcare facilities. PARTICIPANTS: Women over 18 years. MAIN OUTCOME MEASURES: Evaluation of the comparative immunophenotype of human breast carcinoma and normal breast tissues. RESULTS: Leukocyte density and specific subgroups of lymphocytes and macrophages were generally higher in breast cancers compared to normal breast tissues. CD3, CD4, CD45RO, CD45RA(2H4), CD45 and HLA Class II (on TIL) were significantly expressed on breast tumour tissues compared with normal tissues (p < .01). Some 30% of T-cells were γδ-TCR positive, but the majority were αβ-TCR in type. CD19 (B-cell), CD14 (FMC32 and 33) and HLA Class I levels (epithelial and TIL) showed no significant differences. IL-2α receptor expression was low or absent on most TIL. CONCLUSIONS: High-sensitivity and image analysis techniques permitted accurate characterisation of the TIL infiltrate for immune profiling. Breast carcinoma showed predominance of CD4 T-cells of mainly memory phenotype. Normal breast tissues showed low leukocyte infiltration. Further correlation of these findings with clinical outcome, including survival, is proceeding with encouraging results.
Keywords: breast cancer; tumour-infiltrating leukocytes/lymphocytes; TIL; high sensitivity immunostaining; video image analysis; VIA; cellular quantitation; CD3; CD4; CD8; CD45; CD56; CD16; CD25; HLA
Description: Published September 24, 2015
Rights: © 2015 The Author(s) Creative Commons CC-BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www. creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
RMID: 0030037318
DOI: 10.1177/2054270415603909
Appears in Collections:Surgery publications

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