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Type: Journal article
Title: Isolation via 454 sequencing, and characterisation of microsatellite markers for the Pilbara endemic Acacia atkinsiana (Fabaceae)
Author: Levy, E.
Byrne, M.
Coates, D.
van Leeuwen, S.
McArthur, S.
Macdonald, B.
Gardner, M.
Citation: Conservation Genetics Resources, 2014; 6(3):585-587
Publisher: Springer
Issue Date: 2014
ISSN: 1877-7252
Statement of
Esther Levy, Margaret Byrne, David J. Coates, Stephen J. van Leeuwen, Shelley McArthur, Bronwyn Macdonald, Michael G. Gardner
Abstract: We isolated 11 new polymorphic microsatellite markers from Acacia atkinsiana, a plant species endemic to the Pilbara region in Western Australia. Next generation (454) sequencing was used to identify 28 microsatellite markers that were trialled in individuals across the species range. Characterisation of 11 of these loci in 24 individuals from a single population yielded between two and nine alleles per locus, and observed heterozygosity ranged from 0.042 to 0.958. Four loci that amplified in A. atkinsiana were also successfully amplified and polymorphic in Acacia ancistrocarpa. These loci will be valuable for assessing population genetic structure in A. atkinsiana and identifying seed sources for rehabilitation of disturbed sites.
Keywords: Acacia atkinsiana
454 GS-FLX
Shotgun sequencing
Rights: © Crown Copyright as represented by the Government of Western Australia, Department of Parks and Wildlife 2014
DOI: 10.1007/s12686-014-0146-3
Appears in Collections:Aurora harvest 7
Ecology, Evolution and Landscape Science publications

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